Journal Issue: JAAS Vol. 7 No. 2
4
Issued Date
2557
Resource Type
Language
tha
eng
eng
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application/pdf
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open access
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ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
คณะสัตวแพทยศาสตร์ มหาวิทยาลัยมหิดล
Journal Volume
JAAS Volume 7
(2557)
Articles
การวินิจฉัยและการรักษาโรค Subacute Ruminal Acidosis (SARA) ในโคนม
(2557) เชาวลิต นาคทอง; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาเวชศาสตร์คลินิกและการสาธารณสุข
Subacute ruminal acidosis (SARA) is a common disease in well-managed, high milk yielding in dairy
herds. Monitoring and diagnosing are very sensitive to do its routine for dairy herds. SARA may be affected
to reduce milk production and milk fat in early lactation or mid lactation cows. Diagnostic tools are clinical
sign outcome or ruminal pH (≥ 5.5) and faecal parameters: faecal sieing and faecal lipopolysaccharide
(LPS). Rumenocentesis is a good practice and only recommend method for SARA diagnosis although stomach
tube use is easy but its value data is not correct. The treatment and prevention of SARA is depending on feeding
and management base following to each situation of dairy herds in order to reduce rumen acidosis load such
as used S. bovis vaccine or added sodium bicarbonate.
แนวทางการจัดทำระบบเวชระเบียนสัตว์ป่วยสำหรับรักษาสัตว์ป่วยในพื้นที่นครปฐม
(2557) แสงเทียน อยู่เถา; มหาวิทยาลัยมหิดล. คณะสังคมศาสตร์และมนุษยศาสตร์. สาขาวิชาเวชระเบียน
The veterinary medical record (VMR) study is aimed to explore the preparation of guideline that develops
VMR model for veterinary cares in Nakhonpathom area. The study methodology included between expert
interview, focus group discussion, and observation. Total ten experts and ten related key-informants were
interviewed. One focus group discussion was set up with 20 participants. Two veterinary hospitals, two pet
clinics, and 2 livestock offices were observed.
The study found that type of animal patients is classified into small pet, wildlife, large animal, and special
or alien animal. In some places, horse and aquatic animals are separated from this classification. According to
VMR system, animal are divides into new animal patient, old animal patient, and admitted animal patient
(in-patient). The detail of pet owner is also recorded with in the system. The VMR is recorded by HN in the
veterinary hospital and by address in the pet clinics. Moreover, the study found that the VMR system of each
veterinary care is not unique. VMR system is not standardized due to lack of personnel such as VMR recorder
(Medical Record Officer), veterinary nurse, public relation officer, and hospital ship as well as the VMR
standard guideline from Veterinarian Council. In addition, the VMR is not indicated in the veterinary hospital
accreditation and the information system is not included in the university veterinary studying program.
The current medical recording program is not covered all needed records in the veterinary hospitals. Therefore,
it is needed to develop VMR processes such as identification, operation, laboratory relation, personnel, the
connection with veterinary council, VMR duplication, VMR standard, training, and information system for
each type of veterinary cares such as veterinary hospital, pet clinics, and livestock agency.
This study suggests that the veterinary cares in Nakhonpathom area should bring the international
VMR system or the worldwide accepted VMR guideline to record the animal information. However, the VMR
system must be applied in consistent with type and size of each veterinary cares. The recommendation for
future study includes the development of VMR pattern, specialist personnel, medical record standard,
and program for animal patient information system in terms of operation and standard.
การแสดงออกของรีคอมบิแนนท์โปรตีนเปลือกนอกของเชื้อไวรัสโรคขนและจงอยปากผิดปกติโดยใช้ระบบเชื้อยิสต์ Pichiapastoris
(2557) ลดาวัลย์ สาริยา; ปรุศก์ สุกใส; อดิศักดิ์ ส่งแจ้ง; ภิรมย์ พรมพิราม; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ศูนย์เฝ้าระวังและติดตามโรคจากสัตว์ป่า สัตว์ต่างถิ่นและสัตว์อพยพ
Psittacine beak and feather disease virus (BFDV) is a haemagglutination circo virus causing psittacine
beak and feather disease (PBFD) in psittacine birds. The infected birds showed feather dystrophy and
occasionally associated with beak deformities. Development of vaccine and detection of the disease is difficult
and ineffective due to this virus could not be cultured in tissue or cell lines and embryonated eggs. The aim of
this study was to express recombinant capsid protein of BFDV using Pichia pastoris system. The recombinant
protein was purified by affinity binding with Ni-NTA resin. From the results, the time point of protein expression
was 48 hours after inducing with methanol. The recombinant capsid protein was expressed in inclusion bodies
form. From SDS-PAGE analysis, the protein had molecular weight of 38 kDa. Moreover, Western blot analysis
demonstrated that this recombinant protein could react with convalescent serum from BFDV-infected bird.
Based on this information, it would be a potential to develop in house BFDV capsid protein-based diagnostic
test and vaccine in the future.
ค่าทางโลหิตวิทยาและเคมีคลินิกในเลือดแมวที่มีสุขภาพดี
(2557) ปริยากร จายคำ; พงษ์พันธุ์ สุวรรณชาติ; ศุภกิจ บัวมาศ; อรุณี แจ้งแสงทอง; วราภรณ์ แก้วคงจันทร์; วชิร ตระกูลชัยศรี; ชุติเพ็ญ บูรณะสินทรัพย์; มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์
Hematology and clinical blood chemistry values are important for evaluate the health of animals,
diagnostic and prognosis. Animals that have any health problem will have different in hematological and clinical
blood chemistry value from that of standard values. However, most standard values laboratory in Thailand
come from foreign countries. The different in strain (breeds), environmental and food may affect hematology
and clinical blood chemistry. To establish the normal hematology and clinical blood chemistry value in our
laboratory, the authors collect the blood from healthy feline that visited Prasuarthon hospital, Faculty of
Veterinary Science, Mahidol University. Hematology and clinical blood chemistry were analyzed by automated
hematology and clinical chemistry analyzer. WBC differential count was performed using blood smear
staining with Modified-Wright's Geimsa. Clinical blood chemistry analyzed compose of blood urea nitrogen
(BUN), Creatinine (Crea), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), alkaline
phosphatase (ALP) and total protein (TP). We found that hematology and blood clinical chemistry has normal
distribution except eosinophil, basophil and monocyte. In addition, we also found that strain (breeds), age and
sex have significant affect to some hematology and clinical blood chemistry.
Fatty acid composition in adipose tissues of goats and sheep reared on either grazing or confinement regimen
(2014) Surasak Jittakhot; Kunaporn Homyog; Samatchaya Pengdit; Chowalit Nakthong; Mahidol University. Faculty of Veterinary Science
Essential fatty acids play a part in many metabolic processes such as immune system, and cardiovascular
system. A lower ratio of omega-6 to omega-3 fatty acids or increased omega-3 fatty acids in human diet was
more desirable in reducing the risk of the chronic diseases. Hence, the objective of this study was to determine
fatty acid composition from goat and sheep adipose tissues with two different rearing regimens, grazing and
confinement. Ten young male crossbred goats and sheep were equally divided into two groups by their species
reared on either grazing or confinement. Analysis of data as a 2 x 2 factorial combination in Completely
Randomized Design showed that different species did affect fatty acid compositions and no significant
differences were found by either rearing regimens in fatty acid composition in perirenal adipose tissues. The ratio
of the saturated to unsaturated fatty acid was significantly higher (P<0.01) in goats than that found in sheep.
But, the effects of rearing and species-rearing interaction were not observed on this ratio. Moreover, there was
significantly higher (P<0.05) α-linolenic acid (C18:3 ω3) in sheep adipose tissue than goats in both rearing
regimens. Thus, ω6/ω3 ratio tended to be lower in sheep than goats. Perirenal adipose tissues from goat and
sheep contained 4.02 % and 4.57 % polyunsaturated fatty acids (PUFA), respectively.
Title
JAAS Vol. 7 No. 2
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์