Publication: Urinary markers in Babesia canis vogeli-infected dogs
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1
Issued Date
2016
Resource Type
Language
eng
ISSN
1906-2257
Rights
Mahidol University
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.9, No.1 (2016), 25-36
Suggested Citation
Sivapong Sungpradit, Rapeeporn Pikhroh, Wanvisa Thanasaksakul Urinary markers in Babesia canis vogeli-infected dogs. Journal of Applied Animal Science. Vol.9, No.1 (2016), 25-36. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15251
Research Projects
Organizational Units
Authors
Journal Issue
JAAS Vol. 9 No. 1
(2559)
Thesis
Title
Urinary markers in Babesia canis vogeli-infected dogs
Alternative Title(s)
ตัวบ่งชี้ในปัสสาวะของสุนัขที่ติดเชื้อ Babesia canis vogeli
Abstract
Canine babesiosis, a life-threatening tick-borne blood parasitic disease in dogs, caused by Babesia canis
vogeli, is a health problem in companion animals. The disease causes febrile illness, hemolytic anemia, pre-hepatic
jaundice, and thrombocytopenia. Moreover, renal dysfunction from babesiosis has been reported. The purpose
of this study was to investigate urinary markers that might be sensitive and specific for the early detection of
renal dysfunction in B. c. vogeli-infected dogs. Blood and urine samples were collected from 11 dogs. The blood
and urine samples were divided into two groups. B. c. vogeli-infected dogs group including six infected dogs
as confirmed by microscopic examination and multiplex polymerase chain reaction. Non-infected dogs group
included five healthy dogs. Blood samples were subjected to hematology and biochemistry analysis while
urine samples were stored at -80oC until analyzed. Three candidate urinary markers (urinary immunoglobulin G,
uIgG; urinary C-reactive protein, uCRP; and urinary retinol-binding protein, uRBP) were examined using
commercial enzyme-linked immunosorbent assays (ELISA); two additional candidate markers, aspartate
aminotransferase to alanine aminotransferase ratio (AST/ALT) and urinary creatinine to serum creatinine
ratio (UCr/SCr) were also studied. The results demonstrated that hemoglobin, red blood cell count,
and hematocrit were significantly different between B. c. vogeli-infected dogs and non-infected dogs while the
candidate markers were not. In conclusion, the selected candidate markers could not be used as urinary
markers for renal dysfunction in B. c. vogeli-infected dogs. However, further study should investigate other
urinary markers such as albumin, tubular enzymes, and tubular proteins as well as high-throughput
technologies such as the proteomic approach.