Publication: Role of a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation
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Issued Date
2010
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eng
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Mahidol University
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Springer Link online
Bibliographic Citation
Journal of Microbiology. Vol.48, No.1 (2010), 63-70.
Suggested Citation
Suda Tunpiboonsak, Rungrawee Mongkolrob, Kaniskul Kitudomsub, Phawatwaristh Thanwatanaying, Witcha Kiettipirodom, Yanin Tungboontina, Sumalee Tungpradabkul Role of a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation. Journal of Microbiology. Vol.48, No.1 (2010), 63-70.. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40221
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Title
Role of a Burkholderia pseudomallei polyphosphate kinase in an oxidative stress response, motilities, and biofilm formation
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Abstract
Burkholderia pseudomallei, a motile and rod Gram-negative bacterium, is the causative agent of melioidosis. The bacterium is an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic infection inside a host. We report here a role of B. pseudomallei polyphosphate kinase in virulence, such as an oxidative stress response, motilities and biofilm formation. The polyphosphate kinase (ppk) mutant is
susceptible to hydrogen peroxide in an oxidative stress condition, unable to perform swimming, swarming motilities, and has lower density biofilm forming capacity than the wild-type strain. We also demonstrated that both polyphosphate kinase and motile flagella are essential and independently involved in biofilm formation. The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile species, are shown to produce higher density biofilm formation than the ppk mutant, but less than wild type B. pseudomallei. © 2010 The Microbiological Society of Korea.