Publication: Schistosoma Mekongi: the in vitro effect of praziquantel and artesunate on the adult fluke.
Issued Date
2006
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eng
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Mahidol University
Bibliographic Citation
Experimental Parasitology. Vol.113, (2006),16-23
Suggested Citation
Wannee Jiraungkoorskul, Somphong Sahaphong, Prasert Sobhon, suda Riengrojpitak, Niwat Kangwanrangsan, วรรณีย์ จิรอังกูรสกุล, สมพงศ์ สหพงศ์, ประเสริฐ โศภณ, สุดา เรียงโรจน์พิทักษ์, นิวัฒน์ กังวานรังสรรค์ Schistosoma Mekongi: the in vitro effect of praziquantel and artesunate on the adult fluke.. Experimental Parasitology. Vol.113, (2006),16-23. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/8781
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Schistosoma Mekongi: the in vitro effect of praziquantel and artesunate on the adult fluke.
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Abstract
The efficacy and tolerance of 80 μg/ml praziquantel (PZQ) and 40 μg/ml
artesunate (ATS) against adult stage Schistosoma mekongi in vitro were
investigated after 3, 6, 12 and 24 h incubation by monitoring worm
motility and compared tegumental changes using scanning electron
microscopy (SEM). Thirty mice were infected with S. mekongi cercaria
for 49 days. Adult worms were collectedf by perfusion method and
prepared for in vitro study. Contraction and decreased motor activity
were observed after as little as 3 h incubation with PZQ and ATS. Some
of the worms were immobile 12 h after exposure, and died within 24 h.
The tegument of S. mekongi showed severe swelling, vacuolization and
disruption, fusion of the tegumental ridges, collapse and peeling. After
12-24 h incubation, PZQ induced similar but they less severe, tegumental
changes to those observed after exposure to ATS. The direct observation
of the fluke motility and SEM study suggest that ATS is more effective
than PZQ in causing tegumental damge in adult S. mekongi, and provides
a basis for subsequent clinical trials.