Publication: Immunolocalization of cytoskeletal components in the tegument of the 3-week-old juvenile and adult fasciola gigantica.
Issued Date
2006
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eng
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Mahidol University
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Elsevier
Bibliographic Citation
Veterinary Parasitoloty. Vol. 135, (2006), 269-278
Suggested Citation
T. Tansatit., S. Sahaphong., S. Riengrojpitak., V. Viyanant., P. Sobhon. Immunolocalization of cytoskeletal components in the tegument of the 3-week-old juvenile and adult fasciola gigantica.. Veterinary Parasitoloty. Vol. 135, (2006), 269-278. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/8788
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Title
Immunolocalization of cytoskeletal components in the tegument of the 3-week-old juvenile and adult fasciola gigantica.
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Abstract
Components of three cytoskeletal elements, namely, microtubule, intermediate and
actin filaments have been localised in the tegument of the 3-week-old juvenile and
adult Fasciola gigantica by means of immunofluorescence and immunoperoxidase
techniques, using mouse monoclonal anti-alpha-tubulin, anti-cytokeratin antibodies
and biotinylated-phalloidin, respectively. The immunostainings with the above
probes were also performed in adult Schistosoma mansoni for comparison. The
presence of tubulin, indicative of microtubules, was demonstrated in the tegumental
cell bodies, their cytoplasmic processes, and the basal layer of the tegumental
syncytium of F.gigantica. While in S. mansoni, tubulin appeared as vertical lines
stretching across the whole thickness of the syncytium. Cytokeratin, representing one
type of intermediate filaments, was detected in the tegumental cell bodies, their
cytoplasmic processes, tegumental syncytium and apines of F. gigantica.
In contrast, cytokeratin was evident only in the syncytium of S. mansoni, but not in
the spines. Phalloidin, which could bind to actin, a subunit of microfilament, was
detected in the tegumental cell bodies, their processes, and the microtrabecular
network which form the scaffold of the tegumental syncytium of F. gigantica. In S.
mansoni, actin was localized in similar tissues except the syncytium was not stained
while spines exhibited intense staining. In F. gigantica, the presence of microtubules
and actin filaments in the tegumental cells, their processes and in the syncytium could
mediate the movement of secretory granules from the cell bodies towards the basal as
well as the apical layer of the tegument. Cytokeratin filaments may serve to reinforce
the integrity of the tegumental syncytium as well as the spines.