Blood leukocyte transcriptomes in Gram-positive and Gram-negative community-acquired pneumonia
Issued Date
2022-03-01
Resource Type
ISSN
09031936
eISSN
13993003
Scopus ID
2-s2.0-85124729185
Pubmed ID
34446464
Journal Title
European Respiratory Journal
Volume
59
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
European Respiratory Journal Vol.59 No.3 (2022)
Suggested Citation
Pereverzeva L. Blood leukocyte transcriptomes in Gram-positive and Gram-negative community-acquired pneumonia. European Respiratory Journal Vol.59 No.3 (2022). doi:10.1183/13993003.01856-2021 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87362
Title
Blood leukocyte transcriptomes in Gram-positive and Gram-negative community-acquired pneumonia
Author(s)
Other Contributor(s)
Abstract
Background Gram-positive and Gram-negative bacteria are the most common causative pathogens in community-acquired pneumonia (CAP) on the intensive care unit (ICU). The aim of this study was to determine whether the host immune response differs between Gram-positive and Gram-negative CAP upon ICU admission. Methods 16 host response biomarkers providing insight into pathophysiological mechanisms implicated in sepsis and blood leukocyte transcriptomes were analysed in patients with CAP upon ICU admission in two tertiary hospitals in the Netherlands. Results 309 patients with CAP with a definite or probable likelihood (determined by predefined criteria) were included. A causative pathogen was determined in 74.4% of admissions. Patients admitted with Gram-positive CAP (n=90) were not different from those admitted with Gram-negative CAP (n=75) regarding demographics, chronic comorbidities, severity of disease and mortality. Host response biomarkers reflective of systemic inflammation, coagulation activation and endothelial cell function, as well as blood leukocyte transcriptomes, were largely similar between Gram-positive and Gram-negative CAP. Blood leukocyte transcriptomes were also similar in Gram-positive and Gram-negative CAP in two independent validation cohorts. On a pathogen-specific level, Streptococcus pneumoniae and Escherichia coli induced the most distinct host immune response. Conclusion Outcome and host response are similar in critically ill patients with CAP due to Gram-positive bacteria compared with Gram-negative bacteria.