Study on the aggregation states of Amphotericin B with Raman spectroscopy and UV-vis spectroscopy
Issued Date
2019
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
Faculty of Pharmacy Mahidol University
Suggested Citation
Nang Sa Lwae, Nattawut Charoenthai, Satit Puttipipatkhachorn, ณัฐวุฒิ เจริญไทย, สาธิต พุทธิพิพัฒน์ขจร (2019). Study on the aggregation states of Amphotericin B with Raman spectroscopy and UV-vis spectroscopy. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/52394
Title
Study on the aggregation states of Amphotericin B with Raman spectroscopy and UV-vis spectroscopy
Other Contributor(s)
Abstract
Amphotericin B (AmB), the polyene macrolide antifungal agent, formulated as a
micellar system was reported as a cause of nephrotoxicity. The aggregation of AmB plays an
important role on its nephrotoxicity. It is necessary to understand the aggregation behavior of
AmB, because it can help in developing new AmB formulations. Therefore, the aim of this
work was to evaluate the molecular aggregation states of AmB at different molar ratios of AmB
to sodium deoxycholate by Raman spectroscopy and UV-vis spectroscopy. At low
concentrations ranging from 1 x 10-5 M to 1 x 10-7 M, UV-vis spectroscopy could detect the
molecular states of AmB as soluble oligomer, and a monomer at maximum wavelength around
325 nm and 408 nm, respectively. However, UV-vis spectroscopy could not be used to study
the molecular states of AmB at higher concentrations. On the other hand, Raman spectroscopy
could detect remarkable intense peaks relating to molecular structure of AmB at higher
concentrations. Heat treatment at 70°C for 20 min caused the slight blue-shifts in UV peak
around 325 nm, indicating the superaggregates formation. In the Raman spectra, the heat
treatment led to the slight shift of the peak around 1556 cm-1 to lower wavenumber. This result
indicated the change in intermolecular bonding between chromophore parts of AmB molecules
which was related to its aggregation states. In conclusion, both Raman spectroscopy and UVvis
spectroscopy could be used to study the molecular states of AmB in micellar system
depending on its concentration.
Description
The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 77-80