Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia
Issued Date
2023-02-01
Resource Type
ISSN
00489697
eISSN
18791026
Scopus ID
2-s2.0-85143354833
Pubmed ID
36461562
Journal Title
Science of the Total Environment
Volume
858
Rights Holder(s)
SCOPUS
Bibliographic Citation
Science of the Total Environment Vol.858 (2023)
Suggested Citation
Wannigama D.L., Amarasiri M., Hongsing P., Hurst C., Modchang C., Chadsuthi S., Anupong S., Phattharapornjaroen P., Ali Hosseini A.H.R., Fernandez S., Huang A.T., Kueakulpattana N., Tanasatitchai C., Vatanaprasan P., Saethang T., Luk-in S., Storer R.J., Ounjai P., Ragupathi N.K.D., Kanthawee P., Sano D., Furukawa T., Sei K., Leelahavanichkul A., Kanjanabuch T., Hirankarn N., Higgins P.G., Kicic A., Chatsuwan T., McLellan A.D., Abe S. Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia. Science of the Total Environment Vol.858 (2023). doi:10.1016/j.scitotenv.2022.159816 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82945
Title
Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia
Author(s)
Wannigama D.L.
Amarasiri M.
Hongsing P.
Hurst C.
Modchang C.
Chadsuthi S.
Anupong S.
Phattharapornjaroen P.
Ali Hosseini A.H.R.
Fernandez S.
Huang A.T.
Kueakulpattana N.
Tanasatitchai C.
Vatanaprasan P.
Saethang T.
Luk-in S.
Storer R.J.
Ounjai P.
Ragupathi N.K.D.
Kanthawee P.
Sano D.
Furukawa T.
Sei K.
Leelahavanichkul A.
Kanjanabuch T.
Hirankarn N.
Higgins P.G.
Kicic A.
Chatsuwan T.
McLellan A.D.
Abe S.
Amarasiri M.
Hongsing P.
Hurst C.
Modchang C.
Chadsuthi S.
Anupong S.
Phattharapornjaroen P.
Ali Hosseini A.H.R.
Fernandez S.
Huang A.T.
Kueakulpattana N.
Tanasatitchai C.
Vatanaprasan P.
Saethang T.
Luk-in S.
Storer R.J.
Ounjai P.
Ragupathi N.K.D.
Kanthawee P.
Sano D.
Furukawa T.
Sei K.
Leelahavanichkul A.
Kanjanabuch T.
Hirankarn N.
Higgins P.G.
Kicic A.
Chatsuwan T.
McLellan A.D.
Abe S.
Author's Affiliation
Yamagata Prefectural Central Hospital
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Kitasato University Graduate School of Medical Science
Perth Children's Hospital
Curtin University
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Naresuan University
King Chulalongkorn Memorial Hospital
Kasetsart University
Mae Fah Luang University
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Tohoku University
Charles Darwin University
Faculty of Medicine, Chulalongkorn University
Ministry of Higher Education, Science, Research and Innovation
The University of Sheffield
Christian Medical College, Vellore
Telethon Kids Institute
Partner Site Bonn-Cologne
MHESI
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Kitasato University Graduate School of Medical Science
Perth Children's Hospital
Curtin University
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Naresuan University
King Chulalongkorn Memorial Hospital
Kasetsart University
Mae Fah Luang University
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Tohoku University
Charles Darwin University
Faculty of Medicine, Chulalongkorn University
Ministry of Higher Education, Science, Research and Innovation
The University of Sheffield
Christian Medical College, Vellore
Telethon Kids Institute
Partner Site Bonn-Cologne
MHESI
Other Contributor(s)
Abstract
The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.