Effect of dietary chitosan supplement on muscle inflammation and recovery after cardiotoxin-induced injury
4
Issued Date
2015
Copyright Date
2015
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 58 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Physiology))--Mahidol University, 2015
Suggested Citation
Thana Thaweeskulchai Effect of dietary chitosan supplement on muscle inflammation and recovery after cardiotoxin-induced injury. Thesis (M.Sc. (Physiology))--Mahidol University, 2015. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/94080
Title
Effect of dietary chitosan supplement on muscle inflammation and recovery after cardiotoxin-induced injury
Alternative Title(s)
ผลของอาหารเสริมไคโตซานต่อการอักเสบและการฟื้นฟูของกล้ามเนื้อหนูหลังจากได้รับบาดเจ็บจากพิษงู cardiotoxin
Author(s)
Abstract
Chitosan is a natural and non-toxic polymer that has a wide range of pharmaceutical application, including drug carrier, wound dressing and neuroprotective agents. This study aimed to evaluate the effect of chitosan on the inflammatory response and muscle recovery after acute injury. Adult male Wistar rats were randomly divided into 2 major groups: control intact and an injury group. The injury group was further divided into vehicle and chitosan treatment. After 1 week of treatment, injury was induced in tibialis anterior (TA) by cardiotoxin injection. At day 1 and day 3 post-injury, rats were sacrificed and contractile functions of TA muscles were measured. After 1 day post-injury, there was a significant reduction in force production in both the vehicle-treated (~42%) and chitosan-treated (~54%) groups compared to the control. These reduction in force, however, were recovered to control value in both injury groups by day 3 post injury, with chitosan-treated group (~14%) showed better improvement than that in the vehicle-treated group (~23%). This effect was associated with increases damage area and expressions of tumor nuclear factor-a (TNF-a), interleukin-6 (IL-6), interleukin-10 (IL-10), and transforming growth factor- B] (TGF-B]) mRNA level in both injury groups at day 1 post-injury. On day 3 postinjury, however, the level of TNF-α and TGF-β] mRNA were further increased only in the chitosan-treated group, while IL-6 and IL-10 mRNA expressions were unaffected in both injury groups. Taken together, these results suggest that chitosan treatment could enhance the inflammatory response but had a small effect on muscle recovery following cardiotoxin-induced injury.
Description
Physiology (Mahidol University 2015)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Physiology
Degree Grantor(s)
Mahidol University