Quantifying fecal and plasma short-chain fatty acids in healthy Thai individuals
Issued Date
2024-12-01
Resource Type
eISSN
20010370
Scopus ID
2-s2.0-85193585468
Journal Title
Computational and Structural Biotechnology Journal
Volume
23
Start Page
2163
End Page
2172
Rights Holder(s)
SCOPUS
Bibliographic Citation
Computational and Structural Biotechnology Journal Vol.23 (2024) , 2163-2172
Suggested Citation
Manokasemsan W., Jariyasopit N., Poungsombat P., Kaewnarin K., Wanichthanarak K., Kurilung A., Duangkumpha K., Limjiasahapong S., Pomyen Y., Chaiteerakij R., Tansawat R., Srisawat C., Sirivatanauksorn Y., Sirivatanauksorn V., Khoomrung S. Quantifying fecal and plasma short-chain fatty acids in healthy Thai individuals. Computational and Structural Biotechnology Journal Vol.23 (2024) , 2163-2172. 2172. doi:10.1016/j.csbj.2024.05.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98490
Title
Quantifying fecal and plasma short-chain fatty acids in healthy Thai individuals
Corresponding Author(s)
Other Contributor(s)
Abstract
Short-chain fatty acids (SCFAs) are involved in important physiological processes such as gut health and immune response, and changes in SCFA levels can be indicative of disease. Despite the importance of SCFAs in human health and disease, reference values for fecal and plasma SCFA concentrations in healthy individuals are scarce. To address this gap in current knowledge, we developed a simple and reliable derivatization-free GC-TOFMS method for quantifying fecal and plasma SCFAs in healthy individuals. We targeted six linear- and seven branched-SCFAs, obtaining method recoveries of 73–88% and 83–134% in fecal and plasma matrices, respectively. The developed methods are simpler, faster, and more sensitive than previously published methods and are well suited for large-scale studies. Analysis of samples from 157 medically confirmed healthy individuals showed that the total SCFAs in the feces and plasma were 34.1 ± 15.3 µmol/g and 60.0 ± 45.9 µM, respectively. In fecal samples, acetic acid (Ace), propionic acid (Pro), and butanoic acid (But) were all significant, collectively accounting for 89% of the total SCFAs, whereas the only major SCFA in plasma samples was Ace, constituting of 93% of the total plasma SCFAs. There were no statistically significant differences in the total fecal and plasma SCFA concentrations between sexes or among age groups. The data revealed, however, a positive correlation for several nutrients, such as carbohydrate, fat, iron from vegetables, and water, to most of the targeted SCFAs. This is the first large-scale study to report SCFA reference intervals in the plasma and feces of healthy individuals, and thereby delivers valuable data for microbiome, metabolomics, and biomarker research.