Scopus 2024
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Item Metadata only Development of an Electrochemical Aptasensor for Paraquat Detection(2024-01-01) Hongoeb J.; Tantimongcolwat T.; Mukhopadhyay S.C.; Ruankham W.; Phopin K.; Hongoeb J.; Mahidol UniversityParaquat (PQ) is one of the most commonly used herbicides aiming to eliminate undesired weeds in both agricultural and non-agricultural settings. Nevertheless, highly life-threatening effects on human health have raised global awareness. Although several countries have persuaded the ban on PQ utilization, the prolonged persistence in environments still poses the risk of its contamination in natural resources and commodities to human exposure. Therefore, the development of alternative analytical techniques for screening and monitoring residue contamination is needed. Herein, this study proposed an electrochemical aptasensor as a sensing platform for PQ detection. The performance results demonstrated the working range between 1 to 10 μg/mL with the calculated limit of detection at 4.44 μg/mL. A good selectivity against the most coutilized pesticides including diquat and glyphosate was also achieved. The recovery rate in spiked canal water showed a satisfactory outcome at 104.94%, implying a promising alternative method for PQ detection.Item Metadata only In Silico Analysis of Inhibitor Potential of Punicalagin Compound in Pomegranate (Punica granatum) Against NS5 DENV-3 Protein(2024-03-06) Kautsar R.; Rachmawati Y.; Rokhim S.; Sucipto T.H.; Damayanti M.; Ramadhani A.H.; Kautsar R.; Mahidol UniversityIndonesia is one of the Dengue Virus (DENV) endemic areas which are dominated by DENV-2 and DENV-3. Until now, no specific drug therapy has been found to cure Dengue Virus Infection (DVI). Punicalagin is one of the active compounds that have the potential to be used as an antiviral. Unfortunately, not many studies have used punicalagin as a DENV antivirus. This study aims to determine the inhibitory potential of punicalagin compounds against NS5 DENV-3 protein through molecular docking. Molecular docking was performed using AutoDock Tools, ChemDraw, and Discovery Studio Visualizer. The target protein used is NS5 DENV-3 protein with PDB ID code: 4V0Q. The ribavirin compound was used as a positive control. The results obtained show that the punicalagin compound has the ability to attach to target receptors in the C-Terminal domain complex. This docking produces a bond free energy (ΔG) of-6.39 kcal/mol. This result is better than the ΔG of the control compound. Punicalagin's Inhibition Constant (Ki) value also showed better results than ribavirin. So it can be seen that the compound punicalagin effectively inhibits DENV replication and has the potential as a DENV drug candidate.Item Metadata only Molecular cloning and tissue expression analysis of tumor necrosis factor (TNF) gene from Macrobrachium rosenbergii in response to pathogen infections(2024-01-01) Suksangiamkul P.; Choolert C.; Ngueanngam N.; Pasookhush P.; Vaniksampanna A.; Longyant S.; Chaivisuthangkura P.; Suksangiamkul P.; Mahidol UniversityTumor necrosis factor (TNF) is a cytokine that plays essential roles in various physiological pathways, including inflammation and immune responses to microbial infections. Therefore, in this study, we isolated and characterized the full-length TNF gene in Macrobrachium rosenbergii (MrTNF) and investigated the expression of MrTNF against Aeromonas hydrophila and Macrobrachium rosenbergii nodavirus (MrNV) infections. The full-length cDNA of MrTNF had 1830 base pairs (bp), consisting of a 5’ untranslated region (5’-UTR) of 396 bp and a 3’-UTR of 54 bp. MrTNF contained an open reading frame (ORF) of 1380 bp, encoding 459 amino acid residues. The structural analysis of MrTNF revealed a transmembrane domain from positions 21 to 43 and a conserved TNF domain from positions 324 to 446. The MrTNF protein exhibited a high identity of 91.88% compared with MnTNF from Macrobrachium nipponense. The phylogenetic tree analysis revealed that MrTNF was closely related to MnTNF from M. nipponense. The expression level of MrTNF mRNA in healthy prawns exhibited high expression in the intestine, muscle, and stomach. MrTNF was significantly up-regulated in hemocytes, muscle, intestine, and stomach upon A. hydrophila infection. Furthermore, MrTNF in muscle, gills, and hepatopancreas was significantly up-regulated upon MrNV challenge. Molecular docking study indicated that MrTNF may interact with the protruding (P)-domain of MrNV triggering a response in the innate immune system of prawns after viral infection. These findings suggest that MrTNF plays a crucial role in the innate immune system of freshwater crustaceans, particularly in response to Gram-negative bacteria and viral infections.Item Metadata only Rachmaninoff’s Preludes as a Cyclic Work(2024-01-01) Nakagawa E.; Nakagawa E.; Mahidol UniversityFor over 18 years during the period 1892 to 1910, Sergei Rachmaninoff composed the Prelude, Op. 3, No. 2; 10 Preludes, Op. 23; and 13 Preludes, Op. 32. The key organization of Rachmaninoff’s 24 Preludes seems random at first glance, not based on any systematic order, as are the sets of Johann Sebastian Bach’s The Well-Tempered Clavier and Frédéric Chopin’s 24 Preludes, Op. 28. However, they consist of all the different major and minor keys and are uniquely integrated by cyclic manipulation of tonal relationships and thematic ideas. While they share numerous features, such as the use of common tones/chords, stepwise motion, chromatic lines, intervallic motives, and a certain rhythmic pattern, they also present divergent elements, such as bold key changes, irregular inclusion of relative-and parallel-key relationship, and effective contrasts in various aspects in many of consecutive preludes. The set of Rachmaninoff’s 24 Preludes is organized in his own unique, original, and meaningful system and conceptual design, which the author believes is highly successful as an integral, cyclic composition.Item Metadata only The Efficacy of Sample Storage Envelopes in Collecting Scales From the Skin and Nails for Mycology Laboratory Diagnosis of Superficial Fungal Infection(2024-01-01) Matthapan L.; Bunyaratavej S.; Leeyaphan C.; Kobkurkul P.; Hutachoke T.; Wongdama S.; Prasertsook S.; Pongkittilar B.; Panyawong C.; Prasong W.; Plengpanich A.; Matthapan L.; Mahidol UniversityBackground: Fungal cultures are unavailable in many hospitals. The development of an effective sample storage solution for timely transportation would improve management of patients with superficial fungal skin and nail infections. Objectives: This study aimed to evaluate the efficacy of sample storage envelopes to preserve skin and nail samples for timely microscopic examination and culture for superficial fungal infections. Methods: Patients aged 18 years and above with suspected superficial fungal infections were enrolled. The samples were divided into four envelopes. The baseline 20% potassium hydroxide (KOH) examination and fungal culture served as reference points. The stored samples were reexamined on Days 3, 7, 14, and 28. Results: The study included 90 patients with suspected superficial fungal infections (45 skin and 45 nail lesions). Reference KOH examinations showed branching septate hyphae in 36 (80.0%) for skin and 35 (77.8%) for nail infections. Reference fungal cultures were positive for the growth of dermatophyte and nondermatophyte molds in 34 (75.6%) and 28 (62.2%) in skin and nail infections, respectively. Sample storage envelopes maintained 100% sensitivity and specificity for up to 28 days with KOH examination for both skin and nail samples. On Day 28, the fungal culture sensitivity was 70.6% for the skin and 64.3% for the nail samples, with specificities of 100.0% and 88.2%, respectively. Conclusions: Sample storage envelopes effectively maintained diagnostic accuracy for up to 28 days with KOH examination for both skin and nail samples. Given the high specificity even on Day 28 for fungal culture, transferring samples within 28 days remains a reliable practice.Item Metadata only Sadayu for Solo Soprano Saxophone: A Creative Research in Music Composition(2024-07-01) Sookjaeng A.; Sookjaeng A.; Mahidol UniversityThis creative research Sadayu is a new composition for solo soprano saxophone. The piece aims to create a new literature for soprano saxophone, to contribute to the academic field of music in Thailand, and to introduce a contemporary composition that integrates elements of Thai traditional music and culture. Sadayu draws inspiration from a segment of the Ramayana literature in which the large mythical bird named Sadayu obstructs Tossakan who is attempting to abduct Sita. The central concept of the piece is to demonstrate the vibrant colors of the melodic line through the use of contemporary techniques including alternate fingering, bisbigliando, a wide vibrato spanning a quarter of a tone, and rapid passages that incorporate swift changes in pitch collection. Additionally, the composition incorporates techniques from Thai traditional music in its melody, specifically Sabad and Uean. The piece has been successfully accomplished by having been performed internationally and is published by Emerald Music Publications.Item Metadata only Autorepairability of ChatGPT and Gemini: A Comparative Study(2024-01-01) Sriwilailak C.; Higo Y.; Lapvikai P.; Ragkhitwetsagul C.; Choetkiertikul M.; Sriwilailak C.; Mahidol UniversityIn recent years, Automated Program Repair (APR), which focuses on automatically fixing source code without human intervention, has become a hot topic in the field of software engineering, leading to the proposal of various automatic repair techniques. Additionally, Lapvikai et al. introduced a new software quality metric called 'Autorepairability.' Autorepairability is a metric that indicates how easily bugs in the target source code can be fixed using APR techniques. By utilizing Autorepairability, it becomes possible to pre-check whether the program repair techniques will work effectively on the target software and to perform refactoring to improve Autorepairability. However, in the past two to three years, program repair using large language models (LLMs) has become more prevalent, and several studies have revealed that these models exhibit superior repair capabilities compared to traditional APR techniques. In this study, we applied Autorepairability to compare the performance of multiple APR techniques. Specifically, we measured and compared Autorepairability using ChatGPT and Gemini, which are representative large language models, as well as kGenProg, a traditional APR technique. The results demonstrated that Gemini exhibited higher repair capabilities compared to both ChatGPT and the traditional APR technique kGenProg. The five code functionalities that Gemini offers higher Autorepairability scores than ChatGPT include (1) geographic and mathematic operations, (2) validation, comparison, and searching operations, (3) data conversion operations, (4) data extraction and comparison operations, and (5) encoding operations.Item Metadata only DEV-EYE: A Tool for Monitoring Bus Factor Using Commit History(2024-01-01) Kazimoto D.M.; Choetkiertikul M.; Ragkhitwetsagul C.; Sunetnanta T.; Kazimoto D.M.; Mahidol UniversityHigh turnover rates in software development present significant challenges, impacting project continuity, reliability, and quality. The bus factor metric helps quantify and indicate risks associated with key personnel dependencies. Existing tools are designed to calculate the bus factor using information from software project repositories and to determine code ownership. However, given that the bus factor should be monitored over time and the nature of projects varies, a bus factor tool must offer the capability to adjust timelines and customize analysis parameters to accommodate different project constraints. To address these gaps, we introduce DEV-EYE, a tool designed to compute and visualize the bus factor using git commit history. DEV-EYE identifies potential bus factors and offers flexible configuration options, allowing users to adjust parameters such as ownership thresholds and analysis timeframes. Additionally, DEV-EYE enables the comparison of current bus factors with historical data, providing a comprehensive view of project dy-namics. Preliminary evaluations indicate that DEV-EYE is highly promising for real-world applications, emphasizing its role in proactive risk management by identifying critical dependencies and promoting knowledge sharing within teams.Item Metadata only Academic Research in Business and the Social Sciences: A Guidebook for Early Career Researchers(2024-01-01) Moschis G.P.; Moschis G.P.; Mahidol UniversityThis book provides doctoral students, junior faculty and early-career researchers with guidelines, resources and strategies for performing and publishing academic research successfully. It helps increase the productivity of researchers by showing efficient and effective ways to increase research output and publication probability, ranging from manuscript preparation and positioning to working with co-authors and journal reviewers. The author uses research findings, anecdotal evidence and illustrations from his academic career to support his views on strategies and tactics that are required of scholars in order to succeed.Item Metadata only Two new genera and three new species of exceptionally rare and endemic freshwater mussels (Bivalvia, Unionidae) from the Mekong Basin(2024-01-01) Jeratthitikul E.; Sutcharit C.; Prasankok P.; Jeratthitikul E.; Mahidol UniversityTwo new genera and three new species of freshwater mussels in the tribe Pseudodontini (Bivalvia, Unionidae) are described from the Mekong Basin in Thailand based on an integrative taxonomic study involving morphology and multi-locus phylogenetic analyses (mitochondrial COI and 16S, and nuclear 28S genes). The monotypic genus, Lannanaia kokensis gen. et sp. nov., presents unique features of being rather compressed, sub-trigonal in outline with short and high shell, and with a distinct posterior wing. Another new genus, Isannaia gen. nov., is characterized by having a thin and moderately inflated shell, with rhomboidal to ovate outline. It includes two lineages that are genetically separated by 3.54% uncorrected COI p-distance, and are herein described as I. fortunata sp. nov. and I. occultata sp. nov. Phylogenetic analyses further revealed that these two new genera were nested within a clade of subtribe Pseudodontina, and with pairwise uncorrected COI p-distance to other genera ranging from 11.42 to 15.66%. Based on the present data, Lannanaia gen. nov. is known only from the Kok River in the north of Thailand, whereas the two species of Isannaia gen. nov. are restricted to tributaries of the Mekong River in the northeast of Thailand. The discovery of rare and probably endemic freshwater mussels in the Mekong Basin thus again highlights the importance of this region among freshwater biodiversity hotspots of the world.Item Metadata only Human-Robot Interaction in Biopsy Procedures: A Biomimetic Dual-Sheath Needle Design Inspired by Insect Ovipositor Mechanics(2024-01-01) Sivaraman D.; Pillai B.M.; Suthakorn J.; Ongwattanakul S.; Sivaraman D.; Mahidol UniversityThis chapter presents a novel bioinspired dual-sheath needle design for precise targeting and tissue sampling in biopsy procedures within a human-robot interaction framework, inspired by the remarkable ovipositors of insects, such as wood and ichneumon wasps. Through comprehensive modeling techniques such as the Euler-Bernoulli beam theory, Timoshenko beam theory, and experimental vali-dations, biomimetic needle mechanics and kinematics are thoroughly investigated. Simulations evaluated the potential of this approach to improve targeting accuracy, reduce tissue trauma, and optimize procedural outcomes in robot-assisted biopsy interventions. The bioinspired dual-sheath design presents an innovative solution for precise and minimally invasive biopsy sampling by leveraging biomimicry and human-robot interactions.Item Metadata only Numerical Study of Electric Field and Temperature Distribution of Fermented Pork Sausage in Domestic Microwave Oven(2024-01-01) Keangin P.; Srimuang W.; Niamnara T.; Rattanadecho P.; Keangin P.; Mahidol UniversityThe fermented pork sausage (Sai Krok Isan) is a popular local food in Thailand. The fermented sausage is made by mixing steamed rice, pork, and various seasonings, so it must be cooked before eating. The cooking method for fermented pork sausage is usually grilling. The problem of grilling is can cause the outer surface to be cooked before the inner surface. Therefore, microwave heating is an option to solve this problem. Because microwaves can penetrate the inner surface, causing the inside of the food to heat before the outside. The cooked temperature of pork is 72 °C. In this study, a three-dimensional (3D) simulation of microwave heating of the fermented pork sausage is performed, and use finite element method (FEM) in Maxwell's equation and heat transfer equation to study the effects of the microwave power inputs (25 W, 50 W and 100 W) and the heating times (60 s, 180 s and 360 s) on the cooked temperature of fermented pork sausage in domestic microwave oven. It is found that when the microwave power input increases, the temperature value increases. Similarly, as heating time increases, the temperature value also increases. It is found that using the microwave power input of 100 W and heating time of 180 s and 360 s has a temperature of more than 72 °C, which indicates that the fermented pork sausage is cooked. The simulation results of this study can be used to develop and design microwave heating in fermented pork sausage or other foods.Item Metadata only A Computational Analysis of 3D Breast Tissue Model to Simulate Thermal Effects and Tissue Damage due to Microwave Ablation(2024-01-01) Keangin P.; Taypongsak C.; Srimuang W.; Niamnara T.; Rattanadecho P.; Keangin P.; Mahidol UniversityBreast cancer is the most common cancer among women worldwide and significantly impacts both the physical and mental health of women. However, there are many methods of cancer treatment, and one popular method is microwave ablation. Microwave ablation is a cancer treatment that uses microwave energy to treat cancer cells, by delivering microwave energy through an antenna to the target tissue. Microwave energy generates heat, which destroys the cancer cells. The temperature that can destroy cancer cells is over 50 °C. However, the microwave ablation treatment of breast cancer has ethical limitations. This study examines the use of microwave energy for breast cancer treatment with computer simulation by finite element method (FEM). The mathematical model that describes the electromagnetic wave propagation within the breast tissue with Maxwell's equation and the heat generated in the breast tissue from the electromagnetic wave is described by Bioheat equation. This study investigates the effects of the types of microwave antennas, microwave powers, and microwave frequencies for 5 min during microwave ablation. The results show that the ability to destroy cancerous tissue in the breast tissue depends on the types of microwave antennas, microwave powers, and microwave frequencies used. The case with the highest damaged volume in breast tissue is the case of using a double slot microwave antenna, microwave power of 20 W, and microwave frequency of 1800 MHz. The findings from this study can be further developed for microwave ablation treatment in other organs.Item Metadata only Influence of Different Drying Methods of Centella Asiatica Leaf on Drying Kinetics, Specific Energy Consumption, and Equivalent Carbon Dioxide Emissions(2024-01-01) Chawengwanicha P.; Keangin P.; Chawengwanicha P.; Mahidol UniversityCentella asiatica (CA) is a medicinal herb with diverse health benefits. Drying is crucial for preserving its quality and bioactive compounds. However, different drying methods can significantly impact drying kinetics, specific energy consumption (SEC), and equivalent carbon dioxide emissions. The effects of various drying methods on CA leaves, focusing on drying kinetics, specific energy consumption, and equivalent carbon dioxide emissions, are investigated. The different drying methods include hot air drying, freeze drying, and microwave drying are selected. The initial mass of fresh CA leaves is controlled equally of all drying methods. The drying kinetics are determined by observed sample weight throughout the experiment. SEC is obtained via an electrical power monitor for each method based on actual electrical energy used. The equivalent carbon dioxide emissions are assessed by considering the energy consumption. The obtained results reveal that microwave drying method led to the fastest drying and the lowest SEC and equivalent carbon dioxide emissions. These findings enable researchers to develop efficient and sustainable drying practices for CA leaves, ensuring the preservation of its medicinal properties while minimizing environmental impact.Item Metadata only Octanoic Acid and Decanoic Acid Inhibit Tunicamycin-Induced ER Stress in Rat Aortic Smooth Muscle Cells(2024-01-01) Sukketsiri W.; Tipmanee V.; Rungruang P.; Higashihara M.; Sumi T.; Moriyama T.; Zaima N.; Sukketsiri W.; Mahidol UniversityER stress is a crucial factor in the progression of vascular cell diseases. Notably, octanoic acid (OA; C8:0) and decanoic acid (DA; C10:0), prominent components of medium-chain fatty acids (MCFAs), may provide potential health benefits. However, their effects on vascular smooth muscle cells (VSMCs) remain unknown. Given the link between ER stress and vascular cell pathological conditions, the primary goal of this research is to investigate the protective effects of OA and DA against ER stress induction in rat aortic smooth muscle cells (RASMCs). To achieve this objective, RASMCs were pretreated with OA and DA at concentrations of 250 and 500 μM for 24 h. Subsequently, the cells were exposed to 1 μg/mL of tunicamycin, an ER stress inducer, for an additional 24 h. Apoptosis was assessed using DAPI staining, while DCFH2-DA probe was used to measure ROS levels. Furthermore, the gene expression of ER stress markers, such as CHOP, GRP78, ATF4, and eIF2α, as well as contractile markers like αSMA and MYH11, was assessed using real-time reverse transcription polymerase chain reaction. Moreover, the αSMA protein level was measured using immunocytochemistry techniques. The study revealed that OA and DA significantly mitigated cell death caused by tunicamycin, decreased ROS production, and inhibited the gene expression of ER stress markers (CHOP, GRP78, and eIF2α). Notably, OA and DA also inhibited the expression of contractile genes (α-SMA and MYH11) and reduced the number of α-SMA-positive cells in tunicamycin-treated RASMCs. These findings indicate that OA and DA offer protection against ER stress–stimulated cell death and ROS generation in VSMCs, thereby supporting their potential therapeutic applications for safeguarding these cells.Item Metadata only Gunshot Wounds(2024-01-01) Opasanon S.; Chuangsuwanich A.; Opasanon S.; Mahidol UniversityGunshot causes tissue damages by disrupting the tissue, through bleeding, and by permitting entrance of infection. The mechanism of tissue injuries is mixed, blunt, and penetrating trauma injuries. For penetrating trauma, destruction of flesh tissue is due to passing of the bullet through it and the large amount of kinetic energy transferred to the tissue. A high-velocity bullet causes more tissue damage than a low-velocity bullet. The management should include lifesaving, thorough history taking and physical examination and local wound care.Item Metadata only Characterization of hybrid waveguide for Terahertz guidance(2024-11-01) Rattananupong P.; Chitaree R.; Rattananupong P.; Mahidol UniversityIt has been reported that the conventional two-wire waveguide is a good candidate for guiding the Terahertz (THz) wave. However, the waveguide may not be suitable for some practical purposes because of its environmental sensitivity and bulky setup. In this study, we proposed the hybrid waveguide as an alternative THz waveguide. The waveguide structure is mainly composed of transparent dielectric material to a THz wave with a central square of air gap flanked by a pair of copper wires along the waveguide axis. The waveguide is robust due to the mechanical support of the metal wires. At the same time, the dielectric cover can prevent any environmental disturbance that could affect the wave propagation properties. The numerical studies of the proposed waveguide were carried out by commercial software COMSOL Multiphysics, which is based on finite element analysis. The simulation results show that the proposed hybrid waveguide can provide low loss and low dispersion due to the guidance mechanism of the surface plasmon wave propagation similar to the conventional two-wire structure. Using two identical copper wires with the radii, the air hole width, and the center-to-center distance given as 150, 300, and 600 µm, respectively, results in the linearly polarized THz wave confined within the central square of the air gap. The effective refractive index of the proposed waveguide fundamental mode is 1.34 at the operating frequency of 0.2 THz. In addition, high modal energy is confined, and a low absorption loss is achieved.Item Metadata only A Personal Experience and Technical Approaches in Robotic Pancreatic Surgery—Dedicated to Professor Ningxin Zhou(2024-01-01) Wang Z.; Yiengpruksawan A.; Peng C.; Wang Z.; Mahidol UniversityPancreatic surgery is considered one of the most difficult and complicated fields among general surgery.Item Metadata only In Memory of Professor Zhou Ning Xin(2024-01-01) Yiengpruksawan A.; Peng C.; Yiengpruksawan A.; Mahidol UniversityI first met Professor Zhou in 2009 when he and his team from the Second Artillery Hospital in Beijing visited the USA to learn and observe robotic surgery. Prior to the visit, I was contacted by their sponsor and was asked if I would be willing to host them at our hospital where I could share my robotic experience with them and show them our operating room facility. I learned that Professor Zhou was an extremely well-regarded surgeon in China. He was at the time the second in command of the Second Artillery Hospital in Beijing and a Vice President of Chinese Hepatobiliary Surgery Society.Item Metadata only Breaking Barriers: Examining Accessibility Research Within the Realm of Engineering Education Between 1986 and 2023(2024-01-01) Narong D.K.; Narong D.K.; Mahidol UniversityExploration of disability representation within engineering education has been limited. There is also a noticeable absence of dedicated reviews on accessibility despite its potential to enhance inclusivity in engineering education. This bibliometric review of research aims to fill the knowledge gap by analyzing 252 Scopus-indexed publications spanning from 1986 to 2023. The descriptive analysis reveals a growing body of literature, primarily contributed by economically developed countries. Citation analysis identifies influential scholars, institutions, and sources, further enriching our understanding and knowledge of this domain. Moreover, keyword co-occurrence analysis uncovers key topics and research themes, including e-learning, assistive technologies, and learning systems and guidelines. Additionally, the study identifies research fronts indicating a shift toward Industry 4.0-related topics focused on promoting user-centered design, inclusion, equity, and accessible education through the use of cognitive technologies, artificial intelligence, augmented reality, and virtual learning. This study concludes by discussing notable gaps in the knowledge base and future research opportunities in such areas as curriculum designs, pedagogical approaches, Industry 5.0, and systematic integration of accessibility, ultimately aimed at enhancing inclusive education, enriching the learning experience, and improving learning outcomes for all students regardless of their abilities and backgrounds within engineering education.