Preliminary study of isoenzyme in anopheles sundaicus collected from tsunami-effected areas

Abstract

Anopheles sundaicus is an important vector vector of human malaria parasites and is primarily anthropophilic in coastal areas of Thailand. This species is attracting more attention from the Ministry of Public Health owing to its role as a malaria vector and extensively breeding throughout coastline after the occurrence of Tsunami in Phang Nga Province of Thailand. The comparatively study was conducted to determine the characteristic of An. sundaicus population inhabiting the affected and unaffected areas in terms of its dispersal and origin by using electrophoresis technique. The screening of 16 enzyme systems (HK, ADK, ∝-GPDH, FUM, XDH, EST, LDH, MDH, PGM, PGI, AOX, ME, MPI, AMY, ADH, and TO) with comprising 22 loci revealed allelic variation and genetic polymorphism among the population of An. sundaicus. Seven of this, Adk-1, Adk-2, Xdh, Est-3, Mdh-1, Adh, Pgm presented a monomorphic locus and 15 loci; HK, Adk-3, ∝-GPDH, Fum-1, Fum-2, Est-1, Est-2, Ldh, Mdh-2 Pgi, Aox, ME, Mpi, Amy and To showed multiple electromorphic locus in all of studied populations. These results suggested that characteristics of both mosquito populations were likely similar and possibly were from the same origin.