Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation
Issued Date
2023-02-01
Resource Type
eISSN
23065354
Scopus ID
2-s2.0-85149073557
Journal Title
Bioengineering
Volume
10
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bioengineering Vol.10 No.2 (2023)
Suggested Citation
Thummarati P., Laiwattanapaisal W., Nitta R., Fukuda M., Hassametto A., Kino-oka M. Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation. Bioengineering Vol.10 No.2 (2023). doi:10.3390/bioengineering10020211 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81715
Title
Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation
Author's Affiliation
Other Contributor(s)
Abstract
Cell sheet engineering, a scaffold-free tissue fabrication technique, has proven to be an important breakthrough technology in regenerative medicine. Over the past two decades, the field has developed rapidly in terms of investigating fabrication techniques and multipurpose applications in regenerative medicine and biological research. This review highlights the most important achievements in cell sheet engineering to date. We first discuss cell sheet harvesting systems, which have been introduced in temperature-responsive surfaces and other systems to overcome the limitations of conventional cell harvesting methods. In addition, we describe several techniques of cell sheet transfer for preclinical (in vitro and in vivo) and clinical trials. This review also covers cell sheet cryopreservation, which allows short- and long-term storage of cells. Subsequently, we discuss the cell sheet properties of angiogenic cytokines and vasculogenesis. Finally, we discuss updates to various applications, from biological research to clinical translation. We believe that the present review, which shows and compares fundamental technologies and recent advances in cell engineering, can potentially be helpful for new and experienced researchers to promote the further development of tissue engineering in different applications.