Streptococcus suis outbreak caused by an emerging zoonotic strain with acquired multi-drug resistance in Thailand
Issued Date
2023-01-01
Resource Type
eISSN
20575858
Scopus ID
2-s2.0-85148250439
Pubmed ID
36790403
Journal Title
Microbial Genomics
Volume
9
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbial Genomics Vol.9 No.2 (2023)
Suggested Citation
Brizuela J., Kajeekul R., Roodsant T.J., Riwload A., Boueroy P., Pattanapongpaibool A., Thaipadungpanit J., Jenjaroenpun P., Wongsurawat T., Batty E.M., van der Putten B.C.L., Schultsz C., Kerdsin A. Streptococcus suis outbreak caused by an emerging zoonotic strain with acquired multi-drug resistance in Thailand. Microbial Genomics Vol.9 No.2 (2023). doi:10.1099/mgen.0.000952 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81965
Title
Streptococcus suis outbreak caused by an emerging zoonotic strain with acquired multi-drug resistance in Thailand
Other Contributor(s)
Abstract
Streptococcus suis is an emerging zoonotic swine pathogen which can cause severe infections in humans. In March 2021, an outbreak of S. suis infections with 19 confirmed cases of septicemia and meningitis leading to two deaths, occurred in Nakhon Ratch-asima province, Thailand. We characterized the outbreak through an epidemiological investigation combined with Illumina and Nanopore whole genome sequencing (WGS). The source of the outbreak was traced back to a raw pork dish prepared from a single pig during a Buddhist ceremony attended by 241 people. WGS analysis revealed that a single S. suis serotype 2 strain belonging to a novel sequence type (ST) of the emergent Thai zoonotic clade CC233/379, was responsible for the infections. The outbreak clone grouped together with other Thai zoonotic strains from CC233/379 and CC104 in a global S. suis phylogeny and capsule switching events between serotype 2 zoonotic strains and serotype 7 porcine strains were identified. The outbreak strain showed reduced susceptibility to penicillin corresponding with mutations in key residues in the penicillin binding proteins (PBPs). Furthermore, the outbreak strain was resistant to tetracycline, erythromycin, clindamycin, linezolid and chloramphenicol, having acquired an integrative and conjugative element (ICE) carrying resistance genes tetO and ermB, as well as a transposon from the IS1216 family carrying optrA and ermA. This investigation demonstrates that multi-drug resistant zoonotic lineages of S. suis which pose a threat to human health continue to emerge.