Utilizing Quantitative Proteomics to Identify Species-Specific Protein Therapeutic Targets for the Treatment of Leishmaniasis
Issued Date
2022-04-19
Resource Type
eISSN
24701343
Scopus ID
2-s2.0-85128339617
Journal Title
ACS Omega
Volume
7
Issue
15
Start Page
12580
End Page
12588
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Omega Vol.7 No.15 (2022) , 12580-12588
Suggested Citation
Krobthong S., Yingchutrakul Y., Samutrtai P., Hitakarun A., Siripattanapipong S., Leelayoova S., Mungthin M., Choowongkomon K. Utilizing Quantitative Proteomics to Identify Species-Specific Protein Therapeutic Targets for the Treatment of Leishmaniasis. ACS Omega Vol.7 No.15 (2022) , 12580-12588. 12588. doi:10.1021/acsomega.1c05792 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84092
Title
Utilizing Quantitative Proteomics to Identify Species-Specific Protein Therapeutic Targets for the Treatment of Leishmaniasis
Other Contributor(s)
Abstract
Leishmaniasis is a tropical disease caused by Leishmania parasites, which are transmitted through the bites of infected sandflies. We focused on the emergence of leishmaniasis in Thailand caused by a species (Leishmania orientalis). Treatment by chemotherapy is not effective against L. orientalis. Hence, we intended to solve this issue using a proteomics approach to investigate protein profiles and in silico analysis for the identification of antigenic proteins from L. orientalis, Leishmania martiniquensis, and Leishmania donovani. Using principal component analysis (PCA), protein profile comparisons indicated that different species of Leishmania are different at the protein level. Proteomics analysis identified 6099 proteins. Among these proteins, 1065 proteins were used for further analysis. There were 16 proteins that were promising candidates for therapeutic aspects as they were abundantly expressed and common to all species. In silico analysis of protein's antigenicity revealed that eight proteins had the potential for the development of antigenic molecules. Protein profile information and these antigenic proteins may play key roles in the pathogeny of leishmaniasis and can be used as novel therapeutic targets against leishmaniasis in the future.