Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation
1
Issued Date
2022-10-01
Resource Type
eISSN
14203049
Scopus ID
2-s2.0-85139862787
Pubmed ID
36235233
Journal Title
Molecules
Volume
27
Issue
19
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecules Vol.27 No.19 (2022)
Suggested Citation
Min T.Z.M.M.M., Phanabamrung S., Chaisriratanakul W., Pankiew A., Srisuwan A., Chauyrod K., Pongskul C., Promptmas C., Leelayuwat C. Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation. Molecules Vol.27 No.19 (2022). doi:10.3390/molecules27196697 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86832
Title
Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation
Other Contributor(s)
Abstract
This work demonstrates the ability of the Ion-Sensitive Field-Effect Transistor (ISFET)-based immunosensor to detect antibodies against the human leukocyte antigen (HLA) and the major histocompatibility complex class-I-related chain A (MICA). The sensing membrane of the ISFET devices was modified and functionalized using an APTES-GA strategy. Surface properties, including wettability, surface thickness, and surface topology, were assessed in each module of the modification process. The optimal concentrations of HLA and MICA proteins for the immobilization were 10 and 50 μg/mL. The dose-response curve showed a detection range of 1.98–40 µg/mL for anti-HLA and 5.17–40 µg/mL for anti-MICA. The analytical precision (%CV) was found to be 10.69% and 8.92% for anti-HLA and -MICA, respectively. Moreover, the electrical signal obtained from the irrelevant antibody was considerably different from that of the specific antibodies, indicating the specific binding of the relevant antibodies without noise interference. The sensitivity and specificity in the experimental setting were established for both antibodies (anti-HLA: sensitivity = 80.00%, specificity = 86.36%; anti-MICA: sensitivity = 86.67%, specificity = 88.89%). Our data reveal the potential of applying the ISFET-based immunosensor to the detection of relevant anti-HLA and -MICA antibodies, especially in the field of kidney transplantation.