CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)
Issued Date
2023-02-01
Resource Type
ISSN
18735061
eISSN
18767753
Scopus ID
2-s2.0-85144418032
Pubmed ID
36528979
Journal Title
Stem Cell Research
Volume
66
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research Vol.66 (2023)
Suggested Citation
Khampang S., Jiamvoraphong N., Laowtammathron C., Lorthongpanich C., Klaihmon P., Srisook P., Kang X., Luanpitpong S., U-pratya Y., Issaragrisil S. CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1). Stem Cell Research Vol.66 (2023). doi:10.1016/j.scr.2022.102990 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81676
Title
CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)
Author's Affiliation
Other Contributor(s)
Abstract
Yes-associated protein (YAP), an important effector protein of the Hippo signaling pathway, acts as a molecular switch in controlling cell proliferation and apoptosis. In this study, a YAP-targeted isogenic sub-clone of the MUSIe002-A was generated, designated as MUSIe002-A-1. The MUSIe002-1 cell line had normal pluripotent stem cell characteristics and karyotype. Its ability to differentiate into three germ layers was confirmed. As reduction of YAP does not disturb the pluripotency of hESCs, this cell line serves as a valuable model to extrapolate the functional role of YAP in stem cell biology and its applications.