Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility
Issued Date
2023-01-01
Resource Type
eISSN
20013078
Scopus ID
2-s2.0-85145645521
Pubmed ID
36604533
Journal Title
Journal of Extracellular Vesicles
Volume
12
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Extracellular Vesicles Vol.12 No.1 (2023)
Suggested Citation
White R., Sotillo J., Ancarola M.E., Borup A., Boysen A.T., Brindley P.J., Buzás E.I., Cavallero S., Chaiyadet S., Chalmers I.W., Cucher M.A., Dagenais M., Davis C.N., Devaney E., Duque-Correa M.A., Eichenberger R.M., Fontenla S., Gasan T.A., Hokke C.H., Kosanovic M., Kuipers M.E., Laha T., Loukas A., Maizels R.M., Marcilla A., Mazanec H., Morphew R.M., Neophytou K., Nguyen L.T., Nolte-‘t Hoen E., Povelones M., Robinson M.W., Rojas A., Schabussova I., Smits H.H., Sungpradit S., Tritten L., Whitehead B., Zakeri A., Nejsum P., Buck A.H., Hoffmann K.F. Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility. Journal of Extracellular Vesicles Vol.12 No.1 (2023). doi:10.1002/jev2.12298 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82335
Title
Special considerations for studies of extracellular vesicles from parasitic helminths: A community-led roadmap to increase rigour and reproducibility
Author(s)
White R.
Sotillo J.
Ancarola M.E.
Borup A.
Boysen A.T.
Brindley P.J.
Buzás E.I.
Cavallero S.
Chaiyadet S.
Chalmers I.W.
Cucher M.A.
Dagenais M.
Davis C.N.
Devaney E.
Duque-Correa M.A.
Eichenberger R.M.
Fontenla S.
Gasan T.A.
Hokke C.H.
Kosanovic M.
Kuipers M.E.
Laha T.
Loukas A.
Maizels R.M.
Marcilla A.
Mazanec H.
Morphew R.M.
Neophytou K.
Nguyen L.T.
Nolte-‘t Hoen E.
Povelones M.
Robinson M.W.
Rojas A.
Schabussova I.
Smits H.H.
Sungpradit S.
Tritten L.
Whitehead B.
Zakeri A.
Nejsum P.
Buck A.H.
Hoffmann K.F.
Sotillo J.
Ancarola M.E.
Borup A.
Boysen A.T.
Brindley P.J.
Buzás E.I.
Cavallero S.
Chaiyadet S.
Chalmers I.W.
Cucher M.A.
Dagenais M.
Davis C.N.
Devaney E.
Duque-Correa M.A.
Eichenberger R.M.
Fontenla S.
Gasan T.A.
Hokke C.H.
Kosanovic M.
Kuipers M.E.
Laha T.
Loukas A.
Maizels R.M.
Marcilla A.
Mazanec H.
Morphew R.M.
Neophytou K.
Nguyen L.T.
Nolte-‘t Hoen E.
Povelones M.
Robinson M.W.
Rojas A.
Schabussova I.
Smits H.H.
Sungpradit S.
Tritten L.
Whitehead B.
Zakeri A.
Nejsum P.
Buck A.H.
Hoffmann K.F.
Author's Affiliation
Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPAM)
Facoltà di Farmacia e Medicina
Faculteit Diergeneeskunde
Medizinische Universitat Wien, Zentrum für Pathophysiologie, Infektiologie und Immunologie
Wellcome Trust Centre for Molecular Parasitology
Institute of Biological, Environmental and Rural Sciences
Universidad de Costa Rica
Universidad de la Republica Facultad de Medicina
University of Belgrade
McGill University, Macdonald Campus
University of Pennsylvania School of Veterinary Medicine
Queen's University Belfast
Aarhus Universitet
The University of Edinburgh
Centro Nacional de Microbiologia
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic
Faculty of Medicine, Khon Kaen University
The George Washington University School of Medicine and Health Sciences
Universitat Basel
Swiss Tropical and Public Health Institute (Swiss TPH)
James Cook University
Leids Universitair Medisch Centrum
Mahidol University
Universität Zürich
Jihočeská Univerzita v Českých Budějovicích
Univerzita Karlova, Farmaceutická Fakulta v Hradci Králové
Wellcome Sanger Institute
Universitat de València
College of Medical, Veterinary & Life Sciences
Universidad de Buenos Aires
HCEMM-SU Extracellular Vesicle Research Group
ELKH-SE Immune-Proteogenomics Extracellular Vesicle Research Group
Facoltà di Farmacia e Medicina
Faculteit Diergeneeskunde
Medizinische Universitat Wien, Zentrum für Pathophysiologie, Infektiologie und Immunologie
Wellcome Trust Centre for Molecular Parasitology
Institute of Biological, Environmental and Rural Sciences
Universidad de Costa Rica
Universidad de la Republica Facultad de Medicina
University of Belgrade
McGill University, Macdonald Campus
University of Pennsylvania School of Veterinary Medicine
Queen's University Belfast
Aarhus Universitet
The University of Edinburgh
Centro Nacional de Microbiologia
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic
Faculty of Medicine, Khon Kaen University
The George Washington University School of Medicine and Health Sciences
Universitat Basel
Swiss Tropical and Public Health Institute (Swiss TPH)
James Cook University
Leids Universitair Medisch Centrum
Mahidol University
Universität Zürich
Jihočeská Univerzita v Českých Budějovicích
Univerzita Karlova, Farmaceutická Fakulta v Hradci Králové
Wellcome Sanger Institute
Universitat de València
College of Medical, Veterinary & Life Sciences
Universidad de Buenos Aires
HCEMM-SU Extracellular Vesicle Research Group
ELKH-SE Immune-Proteogenomics Extracellular Vesicle Research Group
Other Contributor(s)
Abstract
Over the last decade, research interest in defining how extracellular vesicles (EVs) shape cross-species communication has grown rapidly. Parasitic helminths, worm species found in the phyla Nematoda and Platyhelminthes, are well-recognised manipulators of host immune function and physiology. Emerging evidence supports a role for helminth-derived EVs in these processes and highlights EVs as an important participant in cross-phylum communication. While the mammalian EV field is guided by a community-agreed framework for studying EVs derived from model organisms or cell systems [e.g., Minimal Information for Studies of Extracellular Vesicles (MISEV)], the helminth community requires a supplementary set of principles due to the additional challenges that accompany working with such divergent organisms. These challenges include, but are not limited to, generating sufficient quantities of EVs for descriptive or functional studies, defining pan-helminth EV markers, genetically modifying these organisms, and identifying rigorous methodologies for in vitro and in vivo studies. Here, we outline best practices for those investigating the biology of helminth-derived EVs to complement the MISEV guidelines. We summarise community-agreed standards for studying EVs derived from this broad set of non-model organisms, raise awareness of issues associated with helminth EVs and provide future perspectives for how progress in the field will be achieved.