Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand
Issued Date
2024-06-01
Resource Type
ISSN
20366590
eISSN
19722680
Scopus ID
2-s2.0-85198241982
Journal Title
Journal of Infection in Developing Countries
Volume
18
Issue
6
Start Page
900
End Page
908
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infection in Developing Countries Vol.18 No.6 (2024) , 900-908
Suggested Citation
Tewawong N., Kowaboot S., Kengkarn S., Thawornwan U., Ramasoota P., Suthienkul O. Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand. Journal of Infection in Developing Countries Vol.18 No.6 (2024) , 900-908. 908. doi:10.3855/jidc.18470 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99741
Title
Characterization of Vibrio parahaemolyticus isolated from clinical specimens and oysters in Thailand
Corresponding Author(s)
Other Contributor(s)
Abstract
Introduction: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand. Methodology: Isolates of V. parahaemolyticus from clinical specimens (n = 77) and oysters (n = 224) were identified by biochemical testing, polymerase chain reaction (PCR) assays, and serotyping. The toxin genes, antimicrobial resistance, and β-lactamase production were determined. Results: A total of 301 isolates were confirmed as V. parahaemolyticus by PCR using specific primers for the toxR gene. The majority of clinical isolates carried the tdh+/trh− genotype (82.1%), and one of each isolate was tdh−/trh+ and tdh+/trh+ genotypes. One isolate from oyster contained the tdh gene and another had the trh gene. Twenty-six serotypes were characterized among these isolates, and O3:K6 was the most common (37.7%), followed by OUT:KUT, and O4:K9. In 2010, most clinical and oyster isolates were susceptible to antibiotics, with the exception of ampicillin. In 2012, clinical isolates were not susceptible to cephalothin (52.4%), streptomycin (95.2%), amikacin (66.6%), kanamycin (61.9%), and erythromycin (95.2%), significantly more frequently than in 2010. More than 95% of isolates that were not susceptible to ampicillin produced β-lactamase enzymes. Conclusions: We found toxin genes in two oyster isolates, and the clinical isolates that were initially determined to be resistant to several antibiotics. Toxin genes and antimicrobial susceptibility profiles of V. parahaemolyticus from seafood and environment should be continually monitored to determine the spread of toxin and antimicrobial resistance genes.