Natural Infection of Two Gourami Species (Trichopodus spp.) With Infectious Spleen and Kidney Necrosis Virus (ISKNV): Clinical, Molecular and Histopathological Findings

dc.contributor.authorDinh-Hung N.
dc.contributor.authorDong H.T.
dc.contributor.authorPhiwsaiya K.
dc.contributor.authorTaengphu S.
dc.contributor.authorLinh N.V.
dc.contributor.authorChatchaiphan S.
dc.contributor.authorRodkhum C.
dc.contributor.authorMai H.N.
dc.contributor.authorDhar A.K.
dc.contributor.authorSenapin S.
dc.contributor.correspondenceDinh-Hung N.
dc.contributor.otherMahidol University
dc.date.accessioned2025-03-19T18:04:41Z
dc.date.available2025-03-19T18:04:41Z
dc.date.issued2025-01-01
dc.description.abstractInfectious spleen and kidney necrosis virus (ISKNV) has a broad host range and poses a significant threat to aquaculture species. Herein, we report two disease outbreaks in snakeskin gourami (Trichopodus pectoralis) in Thailand, marked by skin haemorrhage, scale loss, internal organ discoloration and degeneration and a mortality exceeding 60%. Additionally, three spot gourami (Trichopodus trichopterus) cultured in the same ponds along with snakeskin gourami were found to be affected and tested positive for the virus. Histopathological examination revealed extensive necrosis of haematopoietic tissues in the kidneys and spleen, accompanied by the presence of numerous megalocytic cells in both fish species. Real-time PCR assays, using protocols targeting the major capsid protein (MCP) gene in a broad range of Megalocytivirus genotypes, confirmed the presence of ISKNV in the infected fish. Furthermore, molecular and phylogenetic analyses based on the MCP gene (1362 bp) and the adenosine triphosphatase (ATPase) gene (720 bp) revealed that the ISKNV strains in gourami and other fish hosts were closely related, suggesting possible cross-species transmission. This report extends the host range of ISKNV and highlights the need to prevent the spread of this virus across species boundaries. Further research is needed to understand the pathogenicity and transmission routes of the virus, gain insights into its epidemiology and develop strategies to mitigate disease outbreaks.
dc.identifier.citationJournal of Fish Diseases (2025)
dc.identifier.doi10.1111/jfd.14110
dc.identifier.eissn13652761
dc.identifier.issn01407775
dc.identifier.pmid40013347
dc.identifier.scopus2-s2.0-86000029942
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/106695
dc.rights.holderSCOPUS
dc.subjectAgricultural and Biological Sciences
dc.subjectVeterinary
dc.titleNatural Infection of Two Gourami Species (Trichopodus spp.) With Infectious Spleen and Kidney Necrosis Virus (ISKNV): Clinical, Molecular and Histopathological Findings
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=86000029942&origin=inward
oaire.citation.titleJournal of Fish Diseases
oairecerif.author.affiliationFaculty of Science, Mahidol University
oairecerif.author.affiliationChulalongkorn University
oairecerif.author.affiliationKasetsart University
oairecerif.author.affiliationThailand National Center for Genetic Engineering and Biotechnology
oairecerif.author.affiliationAsian Institute of Technology Thailand
oairecerif.author.affiliationThe University of Arizona
oairecerif.author.affiliationChiang Mai University

Files

Collections