Scopus 2025
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Item Metadata only Punching above their weight? Examining player development pathways in Belgian football: a retrospective analysis(2025-02-03) Bullough S.; Balliauw M.; Mills A.; Fallon N.; Bullough S.; Mahidol UniversityPurpose – Belgium has consistently produced world-class footballers, outperforming many larger football associations despite having a smaller talent pool and fewer resources. This paper aims to (1) examine the development pathways of Belgian professional players over the past decade and (2) quantify the role of opportunities in European leagues outside the “Big 5” for a comparative analysis of different player development systems. By exploring how Belgian players progress through various leagues and systems, this study seeks to identify strategic insights that can inform the development practices of both Belgian football and other European nations with similar constraints. Design/methodology/approach – Data on player performance were collected over ten seasons (2013/14 to 2022/23) from 15 European leagues, including the “Big 5” (England, France, Germany, Italy and Spain) and ten other prominent leagues (Austria, Czech Republic, Denmark, Greece, Netherlands, Poland, Portugal, Scotland, Switzerland and Turkey). The variables collected included player name, nationality, team, league, season, appearances, minutes played, age and position. For Belgian players in the sample, the youth academies they attended were also noted. Findings – Belgian players have achieved significant success, particularly in terms of transitioning into the “Big 5” leagues. However, the number of “home-grown” players making appearances and gaining minutes in the Belgian league is relatively low (ranking 13th of the 15 leagues studied). In contrast, Belgian players rank third in terms of total minutes played in the “Big 5” leagues, behind Portuguese and Dutch players. Anderlecht was the most successful Belgian club in developing professional players, and other key contributors were clubs with consistent Champions League participation. Originality/value – While substantial research exists on player development in the “Big 5” European leagues, comparatively little attention has been given to other significant European leagues. This paper addresses that gap by examining the role of smaller leagues in developing young talent, despite their limited resources and reduced access to Europe's elite competitions. The findings highlight the need for policy reforms and strategic improvements at both the national and European levels to enhance player development pathways.Item Metadata only International consensuses and guidelines on diagnosing and managing cytomegalovirus (CMV) retinitis by the Asia-Pacific Vitreo-retina Society (APVRS), the Asia-Pacific Professors of Ophthalmology (AAPPO) and the Asia-Pacific Society of Ocular Inflammation and Infection (APSOII)(2025-09-01) Hwang D.K.; Ng D.S.C.; Qian Z.; Agrawal R.; Chan A.S.Y.; Chhablani J.; Choopong P.; Gupta V.; Invernizzi A.; McCluskey P.; Lee C.S.; Natarajan S.; La Distia Nora R.; Ocampo V.V.; Singh R.; Somkijrungroj T.; Sonoda K.H.; Wei W.; Wong I.Y.H.; Lam D.S.C.; Tao Y.; Hwang D.K.; Mahidol UniversityWith the paradigm changes in antiviral therapy, there are a myriad of emerging controversies in the management of cytomegalovirus retinitis (CMVR). A certain extent of variability exists in the management of CMVR among clinical practices worldwide. Hence, alignment in the management strategy is important towards optimizing the care of CMVR. An international panel of experts (IPE) formulated consensus statements for CMVR regarding to its 1) diagnosis, 2) screening, 3) treatment, 4) management in special populations and 5) emerging technologies. The clinical diagnosis of CMVR relies on patient’s susceptibility due to compromised immune function and characteristic fundus manifestations. Polymerase chain reaction (PCR) of intraocular fluid for detection of CMV is indicated when confirmation is necessary. Oral valganciclovir is the preferred first-line treatment, and intravitreal ganciclovir injection when CMVR threatens to involve the posterior pole. Cessation of maintenance treatment can be considered after 6 months when CMVR remains inactive with immune reconstitution. Immune recovery uveitis (IRU) must be distinguished from CMVR relapse. Screening is recommended for high risk cases. Utilization of telemedicine and artificial intelligence-aided interpretation will help to alleviate the resources required for CMVR screening. Evidence for novel antiviral and immunotherapy have been appraised as second-line treatment options.Item Metadata only Current treatment practices and efficacy in solar urticaria: insights from a patient survey(2025-01-01) Kiefer L.; Aulenbacher F.; Terhorst-Molawi D.; Giménez-Arnau A.M.; Gonçalo M.; Fukunaga A.; Kocatürk Göncü E.; Kulthanan K.; McSweeney S.; Röckmann H.; Weller K.; Rhodes L.E.; Pereira M.P.; Kiefer L.; Mahidol UniversityBackground: Solar urticaria (SolU) is a rare chronic inducible urticaria and photodermatosis, presenting with wheal/flare formation accompanied by severe itch, following exposure to light in the triggering action spectrum. Therapeutic options remain limited for SolU, and the perspective of patients regarding the efficacy of available treatments remains unknown. Methods: Patients with SolU, organized in a disease-specific Facebook group, were asked to complete an electronic questionnaire on their condition and therapies performed between May 2023 and April 2024. The certainty of SolU diagnosis was differentiated as i) physician confirmed by clinical presentation, ii) light provocation tests, or iii) patient-reported. Study outcomes included clinical presentation, triggering action spectrum, disease severity, impairment of quality of life, therapies performed, and their efficacy. Logistic regression models were used to study the association between clinical factors and treatment outcomes. Results: A total of 112 patients (female, n = 94; median age, 42 years) participated in the study. Most patients considered their condition severe or extremely severe (n = 72, 76.6%) with a very/extremely impacted quality of life (n = 82, 86.3%). The majority of patients received non-sedating antihistamines (58.9%, n = 66), leading to worsening, no change, or only slight improvement in most cases (82.2%, n = 53). Omalizumab was given to 28 patients and induced complete control in 32.1% of cases. Treatments with sedating antihistamines, ciclosporin, systemic corticosteroids, phototherapy, and Polypodium leucotomos were performed in a residual number of patients and did not lead to a substantial improvement of the symptoms. Antihistamines were more effective in patients with mild disease, whereas omalizumab maintained a positive response across different disease severity levels. Conclusion: SolU is generally perceived as severe by affected patients, leading to a high impairment of quality of life. Performed therapies, including off-label treatments, are not sufficient to reach complete remission of symptoms in the majority of patients. Effective therapeutics for SolU are urgently needed to achieve better care for this highly burdened patient population.Item Metadata only Cultivation of Native Tissue-Cultured Kappaphycus alvarezii Plantlets at Multiple Sites in The Myeik Archipelago, Myanmar(2025-10-30) Hlaing W.M.M.; Kitisripanya T.; Jarukamjorn K.; Hlaing W.M.M.; Mahidol UniversityCommercial cultivation of Kappaphycus alvarezii (Cottonii) offers opportunities for Myanmar's coastal economy through sustainable aquaculture and economic development. This study assessed the feasibility of large-scale K. alvarezii cultivation in the Myeik Archipelago using tissue-cultured plantlets. Growth performance was evaluated across three sites-Pyin Htet Aw, Thae Chaung, and Don Pale Aw-and the impact of environmental conditions on growth and productivity was examined. Tissue-cultured K. alvarezii plantlets were cultivated at three sites over five weeks. Growth performance and daily growth rate (DGR), along with environmental parameters including temperature and salinity, were monitored. Carrageenan yield and viscosity were measured to evaluate seaweed quality. Growth performance varied across sites, with DGRs ranging from 9.10 to 9.32%. Don Pale Aw yielded the highest biomass (963.00 ± 336.19 g), while Thae Chaung had the lowest (848.00 ± 152.65 g). Salinity significantly influenced growth and carrageenan yield, with higher salinities (31.25–33.15 PSU) supporting greater productivity. Growth remained stable across the temperature range (27.05–31.35°C), indicating a slight thermal tolerance over the previously established ideal range (27–29°C). The fresh-to-dry weight ratio was consistent across sites, confirming stable biomass conversion efficiency. Tissue-cultured plantlets exhibited strong adaptability to local environmental conditions, supporting the viability of large-scale K. alvarezii farming in Myanmar. Selecting sites with optimal environmental conditions (such as moderate hydrodynamics, stable salinity, high light availability, and favorable temperatures) and combining this with sustainable seaweed farming techniques has the potential to enhance local livelihoods, diversify the economy and advance Myanmar’s aquaculture sector.Item Metadata only Multi-Spring Model and Pushover Analysis of Masonry-Infilled Wall in RC Frame Under Tsunami Loading(2025-09-01) Foytong P.; Thanasisathit N.; Ornthammarath T.; Tirapat S.; Prasomsri J.; Nanongtum A.; Ruangrassamee A.; Chindaprasirt P.; Foytong P.; Mahidol UniversityThis study investigated the behavior of masonry-infilled walls (MIWs) within reinforced concrete (RC) frames when exposed to hydrodynamic forces from tsunamis by employing a multi-spring modeling approach across different inundation levels. The proposed analytical model divided the MIW into 1 to 5 horizontal nonlinear spring elements that were allocated along the wall's height. Each spring represented a segment of MIW and was defined by a tri-linear force– displacement relationship. The model was calibrated with the experimental data from previous studies and was analyzed using pushover assessment under uniformly distributed hydrodynamic forces corresponding to four tsunami inundation levels (0.25H, 0.50H, 0.75H, and 1.00H). The models, which had employed four or five horizontal springs, had most effectively replicated MIW behavior under tsunami loading at all inundation depths. Conversely, single-spring models tend to overestimate lateral resistance by up to 50%, particularly when the frame is only partially submerged. This discrepancy arises because less force is transmitted through the MIW, with a greater amount of it being transferred directly to the foundation. The utilization of several spring elements provided a realistic load path, improved the interaction between the frame and MIW characterization, and optimized the precision in simulating lateral resistance and post-peak behavior.Item Metadata only Does work-family conflict predict allostatic load? A 4-year longitudinal study(2025-12-01) Saiki M.; Loerbroks A.; Li J.; Saiki M.; Mahidol UniversityObjective A growing number of studies have reported that occupational psychosocial factors increase the risk of cardiovascular disease. Allostatic load (AL) is a main biological mechanism that explains the pathway from stress to disease. This study examined whether work-to-family conflict (WFC) and family-to-work conflict (FWC) at baseline would be associated with changes in the AL index (ALI) across four years. Methods We used the Midlife in Japan survey, Wave I, and the biomarker projects, Waves I and II. This study included 152 participants with paid jobs and variables of interest at baseline and target biomarkers at baseline and follow-up. We examined the four-year longitudinal changes in the ALI using two methods: comparing ALI scores at baseline and follow-up, named "Two ALI approach,"and creating one ALI score considering changes at baseline and follow-up, named "One ALI approach."Negative binomial regression analysis and generalized estimating equations were used for analyses. The results were reported by count ratios (CRs) and 95% confidence intervals (CIs). Results In the final model, our results demonstrated that WFC at baseline was significantly associated with increased ALI using the "Two ALI approach"(CR 1.15, 95% CIs: 1.03, 1.28) and "One ALI approach"(CR 1.15, 95% CIs: 1.01, 1.32), whereas FWC did not show statistical significance in both methods. Conclusions WFC was longitudinally associated with increased ALI. It is crucial to mitigate the effects of work-to-family conflict. Key messages What is already known on this topic? Previous studies have documented that occupational psychosocial factors adversely affect workers' cardiovascular health. However, the underlying mechanisms, particularly whether perceptions of work-family conflict influence biomarkers prior to disease onset, are poorly understood. What this study adds? This study found that work-to-family conflict at baseline was significantly associated with increased allostatic load index over 4 years. Family-to-work conflict at baseline was not associated with increased ALI in 4 years. How this study might affect research, practice, or policy? Companies and occupational health providers can use work-to-family conflict and ALI as screening tools to enhance employees' cardiovascular health. Interventions targeting the reduction of work-to-family conflict might improve workers' cardiovascular health.Item Metadata only Administration of green tea polyphenols mitigates iron-overload-induced bone loss in a β-thalassemia mouse model(2025-12-01) Xu H.; Settakorn K.; Khantamat O.; Li J.; Charoenphandhu N.; Chanpaisaeng K.; Paradee N.; Srichairatanakool S.; Koonyosying P.; Xu H.; Mahidol UniversityOsteoporosis is a frequent complication in β-thalassemia patients with iron overload, primarily driven by iron-induced oxidative damage and subsequent bone loss. Strategies that promote iron elimination and mitigate oxidative stress may help slow the progression of osteoporosis. Green tea extract (GTE, Camellia sinensis), enriched in epigallocatechin-3-gallate (EGCG), has both antioxidant and iron-chelating activities. This study assessed the effects of GTE on bone health in β-thalassemia knockout mice subjected to 4 weeks of iron dextran injections, followed by 2 months of daily oral treatment with deionized water, deferiprone (50 mg/kg), GTE (50 mg EGCG/kg), the combination of deferiprone and GTE, EGCG (50 mg/kg), or vitamin D3 (0.5 μg/kg). GTE treatment reduced systemic iron burden, malondialdehyde, alkaline phosphatase, and parathyroid hormone, while improving femoral microarchitecture, bone mineral density, plasma calcium, and bone morphogenetic protein expression. These findings suggest GTE protects against iron-induced bone loss through combined chelation and antioxidation, supporting its potential as a therapeutic strategy.Item Metadata only Clinical Evaluation and Prescription of Insoles(2025-01-01) Nouman M.; Chatpun S.; Nouman M.; Mahidol UniversityPrefabricated insoles offer basic cushioning and minimal customization, suitable for low-risk patients. Effective insoles utilize materials like soft foams (EVA, Poron) for cushioning and redistribution, often in multilayer constructions, combined with firmer materials for structural support. Design features include deep heel cups, arch supports, and metatarsal pads to fine-tune pressure relief. Advanced technologies, including CAD/CAM, 3D printing, artificial intelligence for foot classification, and wearable pressure sensors, are being integrated to enhance precision, personalization, and real-time feedback. Clinical evaluation uses outcome measures like foot ulceration incidence and quantitative plantar pressure metrics, with a key target being substantial peak pressure reduction, ideally keeping pressure below 200 kPa. Successful insole prescription is confirmed by follow-up measurements showing substantial offloading of vulnerable areas.Item Metadata only Beyond Capabilities: Reframing Coercion and Responsibility in Antimicrobial Stewardship(2025-11-01) Rattanapitoon N.K.; Thanchonnang C.; Rattanapitoon S.K.; Rattanapitoon N.K.; Mahidol UniversityItem Metadata only Occupational Therapy Interventions for Auditory Processing Disorder in Children with Autism Spectrum Disorder: A Scoping Review(2025-09-01) Mohd Yasin M.D.; Mohd Bakri A.N.; Mohd Nasir I.A.; Elam B.; Khemthong S.; Che Daud A.Z.; Mohd Yasin M.D.; Mahidol UniversityMany children with ASD experience sensory processing challenges, such as difficulties with auditory processing disorders, affecting their occupational performance. Occupational therapists address these challenges through targeted interventions. This scoping review aimed to present available occupational therapy intervention strategies for auditory processing disorders in children with ASD. Relevant peer-reviewed articles from ScienceDirect, Web of Science, Scopus, Wiley Online Library and Cochrane Library were systematically searched from inception to May 2023. Eight studies that investigated occupational therapy intervention strategies focusing on auditory processing disorder for children with ASD were included based on eligibility criteria. Then, the studies were systematically extracted, organised and categorised according to themes. Three major themes emerged namely: 1) types of intervention, 2) approach of interventions and 3) dosage/intensity of intervention. This review highlights the unique role of occupational therapists in addressing sensory processing challenges, which is feasible to implement for children with ASD.Item Metadata only Dual-Functional Amine-Modified Aluminum-Doped MCM-41 Nanoparticles for Concurrent Zoledronic Acid Adsorption and Geranylgeraniol Delivery for Prevention of Medication-Related Osteonecrosis of the Jaw(2025-11-12) Pichaipanich P.; Singhatanadgit W.; Thavornyutikarn B.; Sungkhaphan P.; Kitpakornsanti S.; Pornsuwan S.; Janvikul W.; Pichaipanich P.; Mahidol UniversityThis study aimed to develop a bifunctional nanomaterial that could simultaneously adsorb zoledronic acid (ZA) and release geranylgeraniol (GGOH) to reverse ZA-induced cytotoxicity. The synthesized aluminum-doped mesoporous silica nanomaterial (AM) was subsequently amine-functionalized by 3-aminopropyltriethoxysilane, generating both amine- and aluminum-containing nanomaterial (NAM), to enhance the ability of nanoparticles to adsorb GGOH. The comprehensive characterization results confirmed the successful aluminum-doping and amine-functionalization of the nanoparticles. The results acquired from both thermogravimetric analysis and high-performance liquid chromatography demonstrated that NAM, rather than AM, served as a good nanocarrier for GGOH loading and controlled-releasing. NAM exhibited up to 12.48% GGOH loading efficiency and GGOH sustained release for over 10 days with a release profile best fitted by the Higuchi model (R2= 0.9868), indicating a diffusion-controlled mechanism. Although AM demonstrated much higher ZA adsorption (>95%), NAM still retained moderate ZA adsorption (∼30%). In vitro assays using RAW 264.7 murine cells revealed that GGOH-loaded NAM was noncytotoxic and completely reversed ZA-induced cytotoxicity and metabolic impairment. Furthermore, it displayed negligible hemolytic activity (<0.5%). The combination of targeted drug delivery and bisphosphonate sequestration via nanostructured silica nanocarriers presents a promising therapeutic approach with translational potential in the prevention of medication-related osteonecrosis of the jaw. The promising cellular results, serving as a preclinical foundation, provide a stepping stone toward in vivo applications.Item Metadata only Personal wellbeing and depression among Thai people living in poverty: evidence from the COVID-19 crisis(2025-12-01) Arsa R.; Meemon N.; Keetawattananon W.; Yenchai D.; Kiewnin K.; Pitong P.; Jangcharoenjittkul K.; Waleewong O.; Arsa R.; Mahidol UniversityBackground: Understanding the multifaceted impacts of global crises on vulnerable populations remains critical. This cross-sectional study examines associations between individual and contextual characteristics, personal wellbeing, and depressive symptoms among individuals living below the national poverty line, utilizing secondary data collected in 2021 during the COVID-19 pandemic. Methods: A structural equation model was applied to examine how socio-demographic characteristics, economic conditions, and health behaviors were associated with mental health outcomes, estimating both direct and indirect pathways between personal wellbeing and depressive symptoms. Results: Among the 694 participants, 12.8% reported mild to moderate depressive symptoms. Older individuals and those who experienced changes in their work status during the pandemic reported lower levels of wellbeing and higher levels of depressive symptoms. These patterns may reflect heightened socioeconomic and psychosocial challenges faced by people living in poverty during crisis conditions. Additionally, individuals living in municipal areas and those with lower physical activity levels were more likely to report depressive symptoms. Although the study was conducted during the pandemic period, the findings illustrate how structural disadvantages and behavioral factors were associated with mental wellbeing among people living in poverty. Conclusions: These findings remain relevant for informing mental health and social protection strategies in future public health or economic crises.Item Metadata only Posthandling Spectral Information Enhancement for Single Cell Raman Molecular Mapping Analysis(2025-11-18) Raj A.; Wattanavichean N.; Kawamukai M.; Yamamoto T.; Hamaguchi H.o.; Raj A.; Mahidol UniversityBiochemical analysis of living systems such as single cells benefits greatly from the label-free and low-invasive molecular mapping with Raman microspectroscopy. Sets of Raman spectra at different spatial points are analyzed to generate Raman molecular maps corresponding to specific chemical species. However, human error and subjective data analysis can be technical issues that limit interpretation and its validity. Here, we present an objective data analysis scheme for postprocessing large data sets of Raman spectra for molecular mapping of living cells. The process comprises three steps: (i) Denoising the spectral data set using low-rank approximation; (ii) obtaining an objective background from data points outside the target cell; (iii) subtracting the thus obtained background using Hypothetical Addition Multivariate Analysis with Numerical Differentiation (HAMAND) via an automatically determined coefficient. Through the present analysis, minor Raman peaks, as indiscernible as they are, can be identified and precisely mapped. We demonstrate a quantitative discussion of cellular components after extracting contributions only from a single target cell from a Raman mapping image where multiple cells or parts of other cells are present. This work opens an improved analysis workflow for accurate spectroscopic analysis of living cells with the advantage of identifying minor Raman peaks unambiguously.Item Metadata only Understanding community perceptions of the importance of ecosystem services from gazetted forests(2025-01-01) Chunwate B.T.; Marchant R.A.; Jew E.K.K.; Stringer L.C.; Chunwate B.T.; Mahidol UniversityUnderstanding how protected areas support forest community livelihoods is crucial for informing forest conservation and reducing land degradation. This paper evaluates ecosystem services perceived by communities near three gazetted forest reserves in Nasarawa State, Nigeria: Odu, Risha, and Doma. We ask three questions: i) What ecosystem services do the community perceive they obtain from the gazetted forests? ii) How important do communities perceive the ecosystem services from the gazetted forest reserves to be to wider society? iii) Are there differences in perceptions across the reserves? Data collection involved multistage sampling across three geopolitical zones, using household surveys, interviews, and focus groups. Data analyses employed SPSS and NVivo. Results indicate gazetted forests provide communities with edible fruits, income, agricultural produce, and climate regulation. Highly valued societal benefits include rainfall, land fertility, biodiversity, and climate regulation. Comparative analysis shows traditional worship is highly valued in Odu but less in Doma and Risha. The study recommends forest management consider community dependence on forest products (e.g. food, building materials, herbs) for health and welfare benefits. Communities could facilitate land use planning and zoning of culturally significant areas and assist in formulating forest policies that address interactions between people and forest use.Item Metadata only CREATING A PERCUSSION TEACHING MODULE FOR PRESCHOOLERS IN CHENGDU(2025-01-01) Fan B.; Tayrattanachai N.O.; Fan B.; Mahidol UniversityChengdu, as the music capital of China, deserves attention for the development of percussion and preschool music education. Currently, when the needs of preschoolers are to gain percussion performance ability so that they can participate in competitions and social Art Examination, teachers can only use teaching materials designed for other age of groups or to rely on their own subjective ideas. Such teaching is unproven； quality and effectiveness cannot be guaranteed, and teaching results vary greatly depending on the individualization of the teacher's situation. The objective of this study was to use the data obtained from the semi-structured interviews in the qualitative research method to create a percussion teaching module for preschoolers in Chengdu. The final outcome of the module consists of two parts. In the training phase, teachers’ training arrangements are given, and the materials were specified. The teaching phase of the module, includes teaching content (beginner level and advanced level), studies and solo pieces, and a parent’s guide. IMPLICATION OF THE THESIS This study creates a teaching module based on the demand of percussion teaching for preschoolers in Chengdu. According to the characteristics of preschool music learning and development, combined with the needs of Chengdu percussion teaching, the gap between popular percussion and classical percussion teaching is solved, and the teaching content and progress are unified. This module provides practical materials for preschooler's percussion teaching in Chengdu, and also provides reference and research basis for future percussion and music education for early childhood.Item Metadata only Insoles for Diabetic Foot Complications(2025-01-01) Nouman M.; Chatpun S.; Nouman M.; Mahidol UniversityA key mechanism is redistributing peak plantar pressure over the plantar surface to prevent excessive load on high-risk areas, especially the forefoot. Custom-made insoles consider individual foot biomechanics to reduce peak plantar pressure. Insoles also absorb and distribute impact loads, enhancing comfort and improving biomechanics. Clinical assessment, involving patient history, gait analysis, and plantar pressure mapping, is crucial for insole prescription, considering factors like foot deformities, mobility, and pressure distribution abnormalities. Insoles are effective in reducing plantar pressure, preventing ulcers, and improving foot function. Both custom-made insole and prefabricated insoles are effective in reducing ulcer risk, though custom-made insole offers tailored support and potentially greater plantar pressure reduction. The benefits extend beyond pressure relief to include significant shock absorption, enhanced gait and stability, and crucial ulcer prevention.Item Metadata only Research Trends and Future Directions in Insole Technology, Foot Care, and AI(2025-01-01) Nouman M.; Chatpun S.; Nouman M.; Mahidol UniversityAdvancements in artificial intelligence, smart materials, smart wearable technology, Internet of Things (IoT)-enabled monitoring systems, computational modeling, and telemedicine are reshaping the landscape of insole technology and diabetic foot care. The integration of real-time data analysis, machine learning, and personalized intervention strategies promises to reduce the burden of diabetic foot complications and enhance patient outcomes. Future research and innovation should focus on scaling these technologies for widespread clinical adoption, addressing challenges in patient adherence, cost-effectiveness, and regulatory approval.Item Metadata only Kinetic analysis of catalytic activity of G-quadruplex/hemin DNAzyme with flanking adenine nucleotides(2025-12-01) Udomprasert A.; Chimasungkanun S.; Kangsamaksin T.; Udomprasert A.; Mahidol UniversityG-quadruplex/hemin DNAzymes are promising nucleic acid catalysts due to their versatility and ease of use in biosensing applications. Their peroxidase-like catalytic activity can be enhanced through various strategies, including modifications to flanking nucleotides. In this study, the catalytic effects of flanking nucleotide modifications at the 3′ and 5′ ends of the DNAzyme were investigated. Additionally, the structural topology of the G-quadruplex/hemin DNAzymes was characterized using circular dichroism (CD) spectroscopy. Similar to the unmodified DNAzyme, the modified G-quadruplex with adenine (A) nucleotide at the 3′-terminal extension adopted a parallel topology in the presence of hemin. After optimizing the reaction conditions, the kinetic parameters of both original and 3′ flanking A modified G-quadruplex/hemin DNAzymes were evaluated using the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of hydrogen peroxide (H2O2). The kinetic analysis revealed a significant enhancement in catalytic efficiency upon the addition of A nucleotides at the 3′ end. Notably, the DNAzyme with a 3′-terminal AA modification exhibited approximately a ten-fold increase in catalytic efficiency compared to the unmodified form, as indicated by higher turnover numbers (kcat) and lower H2O2 substrate affinity (Km). This enhanced catalytic performance was further demonstrated by improved colorimetric signal detection of circulating tumor DNA (ctDNA), underscoring the potential of the modified DNAzymes for more sensitive detection in colorimetric biosensor applications.Item Metadata only Hierarchical Graphene/Au/Polyaniline Nanostructured Electrode for Dual-Modality Electrochemical LAMP Biosensing of Helicobacter pylori(2025-11-18) Kumar R.R.; Wang C.Y.; Bharti A.M.; Hu W.C.; Zhang Y.C.; Yu L.S.; Tungtrakarnkul P.; Wu K.C.W.; Chuang C.H.; Kumar R.R.; Mahidol UniversityHelicobacter pylori (HP) infection is directly associated with over 90% of all gastric cancer (GC) cases. Currently available HP tests are prone to false negatives and are inapt for decentralization. Reliable and user-friendly detection platforms for timely diagnosis and routine monitoring are critical to patient survival. Herein, we targeted this unmet need by developing a novel biosensing platform that strategically combined the robust versatility of electrochemical techniques with the sensitivity and specificity of loop-mediated isothermal amplification (LAMP) to enable the accurate detection of HP at the point-of-care (POC). The biosensor design consisted of an underlying screen-printed carbon electrode (SCPE), sequentially modified with (i) highly conductive acid-functionalized sp2 graphene (Gr) and (ii) needle-like gold microstructures encapsulated with (iii) pH-sensitive polyaniline (PANI), assembled between laser-cut cover layers with a built-in reaction chamber for facile sample handling and detection. LAMP was performed using synthesized primers targeting the HP glmM gene. The amplification inherently generates H+, triggering pH variations, which are precisely tracked by the developed biosensor to simultaneously monitor amplicon growth and quantify HP DNA concentrations via two modes: continuous mode and segmented mode. High sensitivity in a wide linear range (1 to 107 copies/μL) with a limit of detection (LOD) of 1 copy/μL is reported. Furthermore, the excellent correlation with clinical results underscored the practical feasibility of this platform to allow reliable early diagnosis of HP infections and serve as a viable alternative to gastric endoscopy.Item Metadata only Sustainability singularity theory for AI green innovation climate governance and future generations(2025-12-01) Dhar B.K.; Sarkar S.M.; Dhar B.K.; Mahidol UniversityThe accelerating climate crisis demands frameworks that move beyond compliance and incrementalism, offering transformative paradigms of sustainability. This Perspective introduces Sustainability Singularity Theory (SST) as a novel lens to envision the tipping point at which artificial intelligence (AI), green technology innovation, and climate governance converge to generate self-reinforcing sustainability transitions. SST advances the claim that sustainability can emerge as an autonomous paradigm—an ontological shift where human and technological agency co-evolve to secure ecological stewardship and intergenerational justice. Central to this framework is the Sustainability Singularity Index (SSI), defined by four components, AI (artificial intelligence adoption), BI (business innovation), CA (climate action), and R (resistance factors). The SSI functions as a heuristic tool for understanding how feedback loops among these forces accelerate resilience while exposing the ethical risks of technocratic rationalities. Grounded in systems theory, phenomenology, and ecological ethics, SST highlights both the promise and perils of AI-enabled transitions. Illustrative examples include renewable energy optimization, digital circular economy platforms, and sustainable finance, positioned as pathways toward inclusive sustainability futures. The Perspective concludes by outlining ethical, managerial, and policy directions that place moral imagination and green technologies at the heart of humanity’s long-term trajectory toward higher stages of civilizational development and the horizon of a Type 1 civilization.