Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality
Issued Date
2024-11-01
Resource Type
eISSN
20762615
Scopus ID
2-s2.0-85210240946
Journal Title
Animals
Volume
14
Issue
22
Rights Holder(s)
SCOPUS
Bibliographic Citation
Animals Vol.14 No.22 (2024)
Suggested Citation
Khophloiklang V., Chanapiwat P., Kaeoket K. Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality. Animals Vol.14 No.22 (2024). doi:10.3390/ani14223178 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102259
Title
Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality
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Author's Affiliation
Corresponding Author(s)
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Abstract
This study aimed to examine the effects of crude garden cress seed oil (CGCSO) on frozen–thawed boar sperm qualities. Semen ejaculates (n = 12) were collected and further divided into six equal aliquots based on CGCSO concentrations (0, 0.5, 1, 1.5, 2, and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved utilizing the traditional liquid nitrogen vapor technique. Subsequently, semen samples were thawed in a thermos with warm water at 50 °C for 12 s and evaluated for sperm morphology using scanning electron microscopy, sperm motility using a CASA, sperm viability, acrosome integrity, mitochondrial function, MDA level, total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), and catalase (CAT) activity. The results indicated that 1% CGCSO resulted in superior post-thaw sperm characteristics, including enhanced sperm morphology, motility, viability, acrosome integrity, and mitochondrial function. Particularly, the total motile sperm increased by 16.5%, progressive motile sperm increased by 13.0%, viability improved by 15.1%, acrosome integrity increased by 14%, and mitochondrial function improved by 14.1% compared to the control group. CGCSO treatment at 1% and 1.5% exhibited the lowest level of MDA (45.73 ± 11.2 and 45.73 ± 11.3 µmol/L, respectively) compared to the other groups. The CGCSO-supplemented groups showed higher values of TAC, GSH-Px, and CAT than the control group but not significantly.