Intergeneric hybridization of Seidenfadenia mitrata (Rchb.f.) Garay with Ascocentrum spp.
4
Issued Date
2022-04-01
Resource Type
ISSN
05677572
eISSN
24066168
Scopus ID
2-s2.0-85130972764
Journal Title
Acta Horticulturae
Volume
1339
Start Page
139
End Page
145
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Horticulturae Vol.1339 (2022) , 139-145
Suggested Citation
Jitsopakul N., Jenrob Y., Pongket U., Thammasiri K. Intergeneric hybridization of Seidenfadenia mitrata (Rchb.f.) Garay with Ascocentrum spp.. Acta Horticulturae Vol.1339 (2022) , 139-145. 145. doi:10.17660/ActaHortic.2022.1339.19 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87470
Title
Intergeneric hybridization of Seidenfadenia mitrata (Rchb.f.) Garay with Ascocentrum spp.
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Intergeneric hybrids of Seidenfadenia mitrata (Rchb.f.) with Ascocentrum spp. were developed using Seidenfadenia mitrata (Rchb.f.) as a female parent and Ascocentrum spp. including Ascocentrum curvifolium (L.) Schltr. and Ascocentrum miniatum (L.) Schltr., as male parents. Pollinia of Ascocentrum spp. were collected from the flowers after 2 d of blooming and then were pollinated with the Seidenfadenia mitrata (Rchb.f.) flowers by hand-pollination. Results showed that the percentage of successful for pod formation was crosses between Seidenfadenia mitrata (Rchb.f.) × Ascocentrum curvifolium (L.) Schltr. and Seidenfadenia mitrata (Rchb.f.) × Ascocentrum miniatum (L.) Schltr. about 80 and 20%, respectively. Sizes of Seidenfadenia mitrata (Rchb.f.) × Ascocentrum curvifolium (L.) and Seidenfadenia mitrata (Rchb.f.) × Ascocentrum miniatum (L.) Schltr. mature pods were about 3.75 cm in length × 0.50 cm in width and 2.69 cm in length × 0.48 cm in width after 168 and 180 d of pollination, respectively. Pods of two hybrids produced seeds. Seeds of Seidenfadenia mitrata (Rchb.f.) × Ascocentrum curvifolium (L.) and Seidenfadenia mitrata (Rchb.f.) × Ascocentrum miniatum (L.) Schltr. germinated into protocorms after 60 and 40 d of sowing on MS agar medium (1962), respectively. Protocorms developed into shoots and roots after tranfered to modified VW agar medium (1949) supplemented with 150 mL L-1 coconut water, 10 g L-1 sucrose, 2 g L-1 activated charcoal, 7 g L-1 agar, and pH at 5.2. Plantlets of two hybrids were successfully acclimatized and grew in greenhouse conditions.