Baicalein inhibits metastatic phenotypes in nasopharyngeal carcinoma cells via a focal adhesion protein integrin β8
Issued Date
2022-01-01
Resource Type
eISSN
14248247
Scopus ID
2-s2.0-85121655031
Journal Title
Pharmaceuticals
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pharmaceuticals Vol.15 No.1 (2022)
Suggested Citation
Kiatwuthinon P., Janvilisri T., Narkthong T., Ngaokrajang U., Kumkate S. Baicalein inhibits metastatic phenotypes in nasopharyngeal carcinoma cells via a focal adhesion protein integrin β8. Pharmaceuticals Vol.15 No.1 (2022). doi:10.3390/ph15010005 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83937
Title
Baicalein inhibits metastatic phenotypes in nasopharyngeal carcinoma cells via a focal adhesion protein integrin β8
Author's Affiliation
Other Contributor(s)
Abstract
Baicalein, a prominent flavonoid from the indigenous herbal plant Scutellaria baicalensis Georgi, possesses broad-spectrum anticancer activities. However, the biological effects of baicalein on nasopharyngeal carcinoma (NPC) and its underlying mechanisms remain unclarified. Thus, in this study, we examined the effects of baicalein on NPC cell lines and investigated the corresponding molecular mechanism through transcriptome profiling. In the study, four NPC cell lines were treated with various concentrations of baicalein at different time points. Cellular toxicity and proliferative inhibition of baicalein were examined by MTT assay. Metastatic phenotypes of NPC cells were investigated by wound healing, transwell, and adhesion assays. Additionally, microarray experiments were performed to determine the cellular pathways affected by baicalein. The expression and localization of the integrin β8 were validated by western immunoblotting and immunofluorescence. Our results revealed that baicalein exhibited its cytotoxicity and antiproliferative activity on all tested NPC cell lines. It also significantly inhibited metastatic phenotypes at sub-lethal concentrations. Transcriptomic analysis showed that baicalein significantly affected the focal adhesion pathway in NPC, where integrin β8 was greatly diminished. Thus, the present study results suggested that baicalein inhibits the metastatic phenotypes of NPC cells by modulating integrin β8, one of the major molecules in a focal adhesion pathway.