Improved Spectrophotometric Method for Determination of High-Range Volatile Fatty Acids in Mixed Acid Fermentation of Organic Residues
Issued Date
2022-05-01
Resource Type
eISSN
23115637
Scopus ID
2-s2.0-85129916196
Journal Title
Fermentation
Volume
8
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fermentation Vol.8 No.5 (2022)
Suggested Citation
Aramrueang N., Lomwongsopon P., Boonsong S., Kingklao P. Improved Spectrophotometric Method for Determination of High-Range Volatile Fatty Acids in Mixed Acid Fermentation of Organic Residues. Fermentation Vol.8 No.5 (2022). doi:10.3390/fermentation8050202 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83747
Title
Improved Spectrophotometric Method for Determination of High-Range Volatile Fatty Acids in Mixed Acid Fermentation of Organic Residues
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Volatile fatty acids (VFAs) are the important intermediates indicating the stability and performance of fermentation process. This study developed the spectrophotometric method for de-termining high-range VFA concentration in mixed-acid fermentation samples. The performance was compared with the gas chromatography (GC) technique. The calibration curves of the modified method showed linearity over a wide and high concentration range of 250–5000 mg/L for individual C2–C6 VFAs in both linear and branched chains. In order to evaluate the modified method for VFA determination in complex fermentation matrices, fermentation samples produced from acidogenic fermentation of plant materials were spiked with acetic (500–1500 mg/L) and butyric acids (1000 mg/L). The accuracy and precision of the modified method for VFA determination were in the range of 94.68–106.50% and 2.35–9.26%, respectively, comparable to the GC method (94.42–99.13% and 0.17–1.93%). The developed method was applicable to measuring all C2–C6 compounds and VFA concentrations in the fermentation samples and had an acceptable accuracy and precision. The pro-posed method is analytically reliable and offers significant advantages in the rapid determination of VFAs in mixed acid fermentation of organic residues.