Inhibition of hamster sperm capacitation and fertilization by piperine
4
Issued Date
2023
Copyright Date
1989
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 134 leaves. : ill.
Access Rights
restricted access
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Physiology))--Mahidol University, 1989
Suggested Citation
Worapot Sriwattana Inhibition of hamster sperm capacitation and fertilization by piperine. Thesis (M.Sc. (Physiology))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90123
Title
Inhibition of hamster sperm capacitation and fertilization by piperine
Alternative Title(s)
ฤทธิ์ของไปปารีนในการยับยั้งการคาพาซิเตทและการปฏิสนธิของเชื้ออสุจิจากแฮมสเตอร์
Author(s)
Abstract
The effect of piperine of fertilizing ability of hamster spermatozoa was investigated in both in vitro and in vivo systems. In the in vitro system, spermatozoa were incubated in capacitation medium which contained various concentrations of pipeine (5,10,20 or 30 mg%) for 3 hrs. Then they were insuminated with ova and incubated for another 3 hrs. Piperine showed a dose dependent inhibition on per cen fartilization and fertilization index. The fertilization was reduced from 93.60% in control to 3.89% in 30 mg% piperine treated group. When the spermatozoa were preincubated without piperine and further inseminated with ova in the fertilization medium containing piperine, The inhibition on fertilization was observed only at high doses (20-30 mg%). The piperine was also found to inhibit acrosome reaction in a dose dependent manner whereas the per cent motility and patterns of sperm movement were not affected. The mechanism of action of piperine was further explored. A decrease in Ca(++) influx into the spermatozoa during capacitation by piperine was observed. This is thought to be a major cause in inhibiting acrosome reaction and subsequent inhibition on fertilization. Vahillic acid which is one of piperine metabolites, was similarly studied but showed no adverse effect. The influence of piperine on fertilizing ability in vivo was assessed. Administration of piperine to female animals during the periods of superovulatory induction and insemination did not affect fertilization in mice whereas it enhanced fertilization in female hamster. This is probably due to the systemic action of piperine on the relaxation of uterine muscle. The prolong treatment of piperine at doses 50-100 mg/kg BW for 6 weeks to male hamsters produced a reduction on body weight of animals when compared to control group. The weight of liver, testis and seminal vesicle were not altered whereas the kidney weight was slightly increased by piperine. The spermatozoa obtained from piperine-treated hamsters were assessed for their fertilizing ability. A marked decrease in per cent motility of spermatozoa from the piperine-treated group (100 mg/kg BW) during incubation in capacitating medium was observed. However, it did not affect fertilization in vitro at this level of drug treatment. The results suggest that the inhibition of fertilization in vitro by piperine was due to an impairment of acrosome reaction which was a consequence of a decrease in calcium influx into the spermatozoa. The lack of in vivo effect of piperine was probably due to a failure in producting enough amount of piperine to exert such inhibitory action. Moreover,its metabolite, vanillic acid, did not affect the sperm function.
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Physiology
Degree Grantor(s)
Mahidol University