The variability of cell-derived microparticles and the age of healthy blood donors
Issued Date
2026-01-01
Resource Type
ISSN
00075027
eISSN
19437730
Scopus ID
2-s2.0-105028641151
Pubmed ID
41275511
Journal Title
Laboratory Medicine
Volume
57
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Laboratory Medicine Vol.57 No.1 (2026)
Suggested Citation
Lerdwana S., Palasuwan D., Palasuwan A., Noulsri E. The variability of cell-derived microparticles and the age of healthy blood donors. Laboratory Medicine Vol.57 No.1 (2026). doi:10.1093/labmed/lmaf072 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114865
Title
The variability of cell-derived microparticles and the age of healthy blood donors
Author(s)
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
Introduction Cell-derived microparticles that promote coagulation can lead to transfusion-related complications. Although age-dependent changes in hemostasis are known, the impact of donor age on microparticle concentration variability remains largely unexplored. We sought to determine microparticle concentrations and investigate their relationship with donor age. Methods Whole-blood samples were collected from volunteers aged 17 to 60years using K<inf>3</inf>EDTA as an anticoagulant. Donors were allocated to 1 of 5 age groups. Flow cytometric analysis and counting beads were used to determine microparticle concentrations and their origins. Results A cross-sectional study of 394 blood donors revealed a mean (SD) total microparticle count of 25693 (1578), 26956 (976), 26979 (989), 24886 (987), and 271331 (1355) particles/µL in blood donors aged 17 to 20, 21 to 30, 31 to 40, 41 to 50, and 51 to 60years, respectively. Similarly, there were no statistically significant differences in the concentrations of red blood cell (RBC)–derived microparticles, platelet-derived microparticles, or leukocyte-derived microparticles among the donor age groups. Linear regression analysis revealed that the r<sup>2</sup> values between the total microparticle, RBC-derived microparticle, platelet-derived microparticle, and leukocyte-derived microparticle concentrations in whole blood and donor age were less than 0.01. Discussion Our assessment of microparticle concentration across different blood donor age groups revealed age-independent variability in microparticle levels. These findings enhance our understanding of how donor factors influence microparticle values.