An indium-111-labelled membrane-targeted peptide for cell tracking with radionuclide imaging
Issued Date
2022-10-19
Resource Type
eISSN
26330679
Scopus ID
2-s2.0-85141793769
Journal Title
RSC Chemical Biology
Volume
4
Issue
1
Start Page
65
End Page
73
Rights Holder(s)
SCOPUS
Bibliographic Citation
RSC Chemical Biology Vol.4 No.1 (2022) , 65-73
Suggested Citation
Pruller J., Pham T.T., Blower J.E., Charoenphun P., Volpe A., Sunassee K., Mullen G.E.D., Blower P.J., Smith R.A.G., Ma M.T. An indium-111-labelled membrane-targeted peptide for cell tracking with radionuclide imaging. RSC Chemical Biology Vol.4 No.1 (2022) , 65-73. 73. doi:10.1039/d2cb00164k Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83577
Title
An indium-111-labelled membrane-targeted peptide for cell tracking with radionuclide imaging
Author's Affiliation
Other Contributor(s)
Abstract
Cell labelling agents that enable longitudinal in vivo tracking of administered cells will support the clinical development of cell-based therapies. Radionuclide imaging with gamma and positron-emitting radioisotopes can provide quantitative and longitudinal mapping of cells in vivo. To make this widely accessible and adaptable to a range of cell types, new, versatile and simple methods for directly radiolabelling cells are required. We have developed [111In]In-DTPA-CTP, the first example of a radiolabelled peptide that binds to the extracellular membrane of cells, for tracking cell distribution in vivo using Single Photon Emission Computed Tomography (SPECT). [111In]In-DTPA-CTP consists of (i) myristoyl groups for insertion into the phospholipid bilayer, (ii) positively charged lysine residues for electrostatic association with negatively charged phospholipid groups at the cell surface and (iii) a diethylenetriamine pentaacetate derivative that coordinates the γ-emitting radiometal, [111In]In3+. [111In]In-DTPA-CTP binds to 5T33 murine myeloma cells, enabling qualitative SPECT tracking of myeloma cells’ accumulation in lungs immediately after intravenous administration. This is the first report of a radiolabelled cell-membrane binding peptide for use in cell tracking.