Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor
3
Issued Date
2025-03-01
Resource Type
ISSN
18735061
eISSN
18767753
Scopus ID
2-s2.0-85214299343
Journal Title
Stem Cell Research
Volume
83
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research Vol.83 (2025)
Suggested Citation
Bumroongthai K., Yodtup C., Jantapalaboon D., Phairoh P., Suparak S., Dhepakson P., Tubsuwan A., Wongkidakarn S. Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor. Stem Cell Research Vol.83 (2025). doi:10.1016/j.scr.2025.103655 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102945
Title
Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor
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Corresponding Author(s)
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Abstract
Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.