Bumroongthai K., Yodtup C., Jantapalaboon D., Phairoh P., Suparak S., Dhepakson P., Tubsuwan A., Wongkidakarn S. Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor. Stem Cell Research Vol.83 (2025). doi:10.1016/j.scr.2025.103655 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102945
Title
Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor
Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.
