The role of cycle inhibiting factor in burkholderia pseudomallei
1
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Pornpan Pumirat, พรพรรณ ภูมิรัตน์, Onrapak Reamtong, อรภัค เลี่ยมทอง, Narisara Chantratita, นริศรา จันทราทิตย์, Sunee Korbsrisate (2014). The role of cycle inhibiting factor in burkholderia pseudomallei. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63370
Title
The role of cycle inhibiting factor in burkholderia pseudomallei
Abstract
Burkholderia pseudomallei, a causative agent of melioidosis, harbor several
virulence factors crucial for its pathogenesis. A cycle-inhibiting factor
(Cif) is one of bacterial effector molecules produced by B. pseudomallei,
however the role of this protein is still unclear. In our previous study, we constructed a
B. pseudomallei cif mutant to explore the function of Cif in B. pseudomallei infection.
Infection of U937 macrophage with B. pseudomallei revealed that host cell contact
was required for the secretion of Cif. Interestingly, Cif was secreted in the host cell in
a manner dependent on the Bsa type III secretion system. Moreover, the responsibility
of Cif is likely important for B. pseudomallei pathogenesis as we found B. pseudomallei
cif mutant showed significant defects in abilities to induce plaque formation and
cytotoxicity of HeLa cells when compared to the wild type. We further investigated
the host protein expression profiles of infected HeLa cells using mass spectrometry.
Investigation by this technique identified 97 up-regulated and 166 down-regulated
proteins that significantly altered in HeLa cells infected with chbP mutant compared
with that of B. pseudomallei wild type strain. Among these, some are associated
with metabolic process, catalytic activity and signaling pathways. Thus, based upon
our findings, we indicate a requirement of Cif that deliver via Bsa apparatus in B.
pseudomallei infection, and the role of Cif might be involved with regulation of the host
cell expression to alter the host cell functions which benefit for the pathogenesis of B.
pseudomallei.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 211.