Anti-Müllerian hormone levels in serum and testes of male dogs: relations with neuter status and bilateral abdominal cryptorchidism
Issued Date
2023-09-15
Resource Type
ISSN
0093691X
Scopus ID
2-s2.0-85162171364
Journal Title
Theriogenology
Volume
208
Start Page
171
End Page
177
Rights Holder(s)
SCOPUS
Bibliographic Citation
Theriogenology Vol.208 (2023) , 171-177
Suggested Citation
Prapaiwan N., Manee-in S., Thanawongnuwech R., Srisuwatanasagul S. Anti-Müllerian hormone levels in serum and testes of male dogs: relations with neuter status and bilateral abdominal cryptorchidism. Theriogenology Vol.208 (2023) , 171-177. 177. doi:10.1016/j.theriogenology.2023.06.015 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87662
Title
Anti-Müllerian hormone levels in serum and testes of male dogs: relations with neuter status and bilateral abdominal cryptorchidism
Author's Affiliation
Other Contributor(s)
Abstract
Anti-Müllerian hormone (AMH) analysis has contributed to new information in the reproductive endocrinology of domestic animals, due to clinically available diagnostic tools. An accurate and rapid diagnostic method to distinguish between neutered and bilateral abdominal cryptorchid dogs is needed in veterinary practice. Therefore, this study uses an enzyme-linked immunosorbent assay to evaluate the clinical relevance of AMH analysis in peripheral blood as a diagnostic tool for dogs with suspected bilateral abdominal cryptorchidism. The possible alteration of the AMH localization in testicular tissue caused by this pathologic condition was also investigated using immunohistochemistry. Male dogs were divided into three groups of healthy intact (n = 14), healthy castrated (n = 14), and bilateral abdominal cryptorchid (n = 14) dogs. The results demonstrated a higher level of serum AMH in the cryptorchid group compared to the intact group (P < 0.01), while serum AMH levels of all castrated dogs were below the limit of detection (<0.05 ng/mL). Moreover, the percentage of positive AMH immunostaining of the intact group was less than that of the cryptorchid group (P < 0.01). A significantly positive correlation was found between serum AMH concentration and localization in testicular tissues (r = 0.93, P < 0.01). Our findings suggest that AMH levels in the peripheral blood could be used as an alternative and rapid screening method for detecting dogs with abdominal cryptorchidism.