Establishment of Afgekia mahidolae B.L. Burtt & Chermsir in vitro culture and effect of elicitation on its bioactive compounds
Issued Date
2023-01-01
Resource Type
ISSN
14786419
eISSN
14786427
Scopus ID
2-s2.0-85137105220
Pubmed ID
36047978
Journal Title
Natural Product Research
Volume
37
Issue
11
Start Page
1829
End Page
1837
Rights Holder(s)
SCOPUS
Bibliographic Citation
Natural Product Research Vol.37 No.11 (2023) , 1829-1837
Suggested Citation
Traiyasuk W., Komaikul J., Anantachoke N., Kitisripanya T. Establishment of Afgekia mahidolae B.L. Burtt & Chermsir in vitro culture and effect of elicitation on its bioactive compounds. Natural Product Research Vol.37 No.11 (2023) , 1829-1837. 1837. doi:10.1080/14786419.2022.2118740 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82985
Title
Establishment of Afgekia mahidolae B.L. Burtt & Chermsir in vitro culture and effect of elicitation on its bioactive compounds
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Afgekia mahidolae is a rare plant species that possesses antioxidant, antimicrobial, and wound healing properties. This study aimed to establish the in vitro culture of A. mahidolae and investigate the effects of elicitors on their phenolic and flavonoid production, including the antioxidant activities. The established callus was prepared in the form of cell suspension cultures to determine the effect of elicitors. After elicitation for 3 days, A. mahidolae cell suspension cultures treated by 5 µM salicylic acid or 100 mg/L yeast extract exhibited significantly higher levels of total phenolic and total flavonoid content than untreated cultures, which correlated to the antioxidant activities. In addition, the profiles of phenolic and flavonoid compounds in the callus and intact leaves of A. mahidolae were determined by LC-MS, which showed different phytochemicals. The findings of this study may encourage more sustainable development of A. mahidolae cultivation.