West Nile virus can be transmitted within mosquito populations through infectious mosquito excreta
Issued Date
2024-11-15
Resource Type
eISSN
25890042
Scopus ID
2-s2.0-85207791662
Journal Title
iScience
Volume
27
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
iScience Vol.27 No.11 (2024)
Suggested Citation
Hamel R., Narpon Q., Serrato-Pomar I., Gauliard C., Berthomieu A., Wichit S., Missé D., Sofonea M.T., Pompon J. West Nile virus can be transmitted within mosquito populations through infectious mosquito excreta. iScience Vol.27 No.11 (2024). doi:10.1016/j.isci.2024.111099 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101918
Title
West Nile virus can be transmitted within mosquito populations through infectious mosquito excreta
Corresponding Author(s)
Other Contributor(s)
Abstract
Understanding the transmission routes of arboviruses is key to determining their epidemiology. Here, we tested whether West Nile viruses (WNVs) are transmitted through mosquito excreta. First, we observed a high concentration of infectious units per excreta, although viruses were short lived. Second, we showed that virion excretion starts early after oral infection and remains constant for a long period, regardless of mosquito infection level. These results highlight the infectiousness of excreta from infected mosquitoes. Third, we found that both larvae and pupae were susceptible to infection, while pupae were highly permissive. Fourth, we established the proof-of-concept that immature mosquitoes can be infected by infectious excreta, demonstrating an excreta-mediated mode of transmission. Finally, by mathematically modeling excreta-mediated transmission in the field, we demonstrated that WNV can be transmitted within mosquito populations. Our study uncovers a route of transmission for mosquito-borne arboviruses, unveiling mechanisms of viral maintenance in mosquito reservoirs.