Plasmidome in mcr-1 harboring carbapenem-resistant enterobacterales isolates from human in Thailand
Issued Date
2022-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85141521652
Pubmed ID
36351969
Journal Title
Scientific Reports
Volume
12
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.12 No.1 (2022)
Suggested Citation
Boueroy P., Wongsurawat T., Jenjaroenpun P., Chopjitt P., Hatrongjit R., Jittapalapong S., Kerdsin A. Plasmidome in mcr-1 harboring carbapenem-resistant enterobacterales isolates from human in Thailand. Scientific Reports Vol.12 No.1 (2022). doi:10.1038/s41598-022-21836-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86383
Title
Plasmidome in mcr-1 harboring carbapenem-resistant enterobacterales isolates from human in Thailand
Other Contributor(s)
Abstract
The emergence of the mobile colistin-resistance genes mcr-1 has attracted significant attention worldwide. This study aimed to investigate the genetic features of mcr-1-carrying plasmid among carbapenem-resistant Enterobacterales (CRE) isolates and the potential genetic basis governing transmission. Seventeen mcr-harboring isolates were analyzed based on whole genome sequencing using short-read and long-read platforms. All the mcr-1-carrying isolates could be conjugatively transferred into a recipient Escherichia coli UB1637. Among these 17 isolates, mcr-1 was located on diverse plasmid Inc types, consisting of IncX4 (11/17; 64.7%), IncI2 (4/17; 23.53%), and IncHI/IncN (2/17; 11.76%). Each of these exhibited remarkable similarity in the backbone set that is responsible for plasmid replication, maintenance, and transfer, with differences being in the upstream and downstream regions containing mcr-1. The IncHI/IncN type also carried other resistance genes (blaTEM-1B or blaTEM-135). The mcr-1-harboring IncX4 plasmids were carried in E. coli ST410 (7/11; 63.6%) and ST10 (1/11; 9.1%) and Klebsiella pneumoniae ST15 (1/11; 9.1%), ST336 (1/11; 9.1%), and ST340 (1/11; 9.1%). The IncI2-type plasmid was harbored in E. coli ST3052 (1/4; 25%) and ST1287 (1/4; 25%) and in K. pneumoniae ST336 (2/4; 50%), whereas IncHI/IncN were carried in E. coli ST6721 (1/2; 50%) and new ST (1/2; 50%). The diverse promiscuous plasmids may facilitate the spread of mcr-1 among commensal E. coli or K. pneumoniae strains in patients. These results can provide information for a surveillance system and infection control for dynamic tracing.