Optimal Stabilization for Long-Term Storage of Nucleic Acid-Based CRISPR/Cas12a Assay for SARS-CoV-2 Detection
Issued Date
2023-01-01
Resource Type
ISSN
2405609X
eISSN
24056103
Scopus ID
2-s2.0-85158877014
Journal Title
Karbala International Journal of Modern Science
Volume
9
Issue
2
Start Page
187
End Page
196
Rights Holder(s)
SCOPUS
Bibliographic Citation
Karbala International Journal of Modern Science Vol.9 No.2 (2023) , 187-196
Suggested Citation
Saisawang C., Naksith P., Sakdee S., Ketterman A.J., Tuntithavornwat S., Nimsamer P., Mayuramart O., Chantaravisoot N., Pisitkun T., Payungporn S. Optimal Stabilization for Long-Term Storage of Nucleic Acid-Based CRISPR/Cas12a Assay for SARS-CoV-2 Detection. Karbala International Journal of Modern Science Vol.9 No.2 (2023) , 187-196. 196. doi:10.33640/2405-609X.3288 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82648
Title
Optimal Stabilization for Long-Term Storage of Nucleic Acid-Based CRISPR/Cas12a Assay for SARS-CoV-2 Detection
Other Contributor(s)
Abstract
In this long-term storage study, we optimized the lyophilization conditions of each reaction stage of a nucleic acid-based assay for SARS-CoV-2 detection. The stability testing demonstrated that the dried reactions from all 3 steps (cDNA synthesis, isothermal amplification and detection) can be kept at ¡20 °C or 4 °C for up to 6 or 3 months, respectively, whereas, if stored at 25 °C or 37 °C, the reagents only could be stored for a few days without quality loss. This suggests that we can have the dried reactions at ¡20 °C for long-term storage until needed. Moreover, this assay is now simpler to perform as each of the 3 steps now proceeds with pre-mixed regents lyophilized in a single tube for each step.