Antigenic Determinant of Helicobacter pylori FlaA for Developing Serological Diagnostic Methods in Children
Issued Date
2022-12-01
Resource Type
eISSN
20760817
Scopus ID
2-s2.0-85144710536
Journal Title
Pathogens
Volume
11
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pathogens Vol.11 No.12 (2022)
Suggested Citation
Park H.E., Park S., Nizamutdinov D., Seo J.H., Park J.S., Jun J.S., Shin J.I., Boonyanugomol W., Park J.S., Shin M.K., Baik S.C., Youn H.S., Cho M.J., Kang H.L., Lee W.K., Jung M. Antigenic Determinant of Helicobacter pylori FlaA for Developing Serological Diagnostic Methods in Children. Pathogens Vol.11 No.12 (2022). doi:10.3390/pathogens11121544 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83496
Title
Antigenic Determinant of Helicobacter pylori FlaA for Developing Serological Diagnostic Methods in Children
Other Contributor(s)
Abstract
The early diagnosis of Helicobacter pylori infection is important for gastric cancer prevention and treatment. Although endoscopic biopsy is widely used for H. pylori diagnosis, an accurate biopsy cannot be performed until a lesion becomes clear, especially in pediatric patients. Therefore, it is necessary to develop convenient and accurate methods for early diagnosis. FlaA, an essential factor for H. pylori survival, shows high antigenicity and can be used as a diagnostic marker. We attempted to identify effective antigens containing epitopes of high diagnostic value in FlaA. Full-sized FlaA was divided into several fragments and cloned, and its antigenicity was investigated using Western blotting. The FlaA fragment of 1345–1395 bp had strong immunogenicity. ELISA was performed with serum samples from children by using the 1345–1395 bp recombinant antigen fragment. IgG reactivity showed 90.0% sensitivity and 90.5% specificity, and IgM reactivity showed 100% sensitivity and specificity. The FlaA fragment of 1345–1395 bp discovered in the present study has antigenicity and is of high value as a candidate antigen for serological diagnosis. The FlaA 1345–1395 bp epitope can be used as a diagnostic marker for H. pylori infection, thereby controlling various gastric diseases such as gastric cancer and peptic ulcers caused by H. pylori.