Assessment of antibody responses to Anopheles SG6-P1 and Aedes N-term 34 kDa salivary peptides: a randomised human-challenge trial of controlled exposures to vector bites
Issued Date
2026-03-02
Resource Type
eISSN
17417015
Scopus ID
2-s2.0-105035340250
Pubmed ID
41765941
Journal Title
BMC Medicine
Volume
24
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Medicine Vol.24 No.1 (2026)
Suggested Citation
Chaumeau V., Kearney E.A., Wasisakun P., Sawasdichai S., Aung A.A., Agius P.A., Min T.H., da Silva Gonçalves D., O'Flaherty K., Rouers A., Aryalamloed S., Htoo G.N., Sue M.P., Tha N.M., Chanida N., Gornsawun G., Chotirat S., Simpson J.A., Rénia L., Nosten F., Fowkes F.J.I. Assessment of antibody responses to Anopheles SG6-P1 and Aedes N-term 34 kDa salivary peptides: a randomised human-challenge trial of controlled exposures to vector bites. BMC Medicine Vol.24 No.1 (2026). doi:10.1186/s12916-026-04732-z Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116241
Title
Assessment of antibody responses to Anopheles SG6-P1 and Aedes N-term 34 kDa salivary peptides: a randomised human-challenge trial of controlled exposures to vector bites
Author's Affiliation
Monash University
Deakin University
Nuffield Department of Medicine
Lee Kong Chian School of Medicine
Faculty of Tropical Medicine, Mahidol University
School of Biological Sciences
Burnet Institute
Mahidol Oxford Tropical Medicine Research Unit
Centre for Epidemiology and Biostatistics
A-Star, Infectious Disease Lab
Deakin University
Nuffield Department of Medicine
Lee Kong Chian School of Medicine
Faculty of Tropical Medicine, Mahidol University
School of Biological Sciences
Burnet Institute
Mahidol Oxford Tropical Medicine Research Unit
Centre for Epidemiology and Biostatistics
A-Star, Infectious Disease Lab
Corresponding Author(s)
Other Contributor(s)
Abstract
BACKGROUND: Human antibodies against mosquito salivary proteins are proposed as proxy biomarkers of exposure to vector bites. This trial sought to characterise the boosting and decay dynamics of antibodies against Anopheles SG6-P1 and Aedes N-term 34kDa salivary peptides in a human challenge model of controlled exposure to the main Southeast Asian malaria and global dengue vectors. METHODS: In this single-centre, open-label, randomised, exploratory factorial trial, healthy volunteers aged 18-60 years with no history of recent travel to rural areas were recruited in Mae Sot, Thailand (ClincalTrials.gov: NCT04478370). Participants were randomly assigned to receive either 35 or 305 bites of mosquitos of laboratory-adapted colonies of Anopheles dirus, Anopheles maculatus, Anopheles minimus, Aedes aegypti and Aedes albopictus using a block randomisation schedule. Samples were collected weekly before, during and after the challenges for 16 weeks. The primary endpoint was total IgG antibodies against Anopheles SG6-P1 peptides measured using high-throughput ELISA and analysed with generalised estimating equations. Outcome assessors were masked to the intervention groups. RESULTS: Between January 21, 2021, and May 10, 2022, 248 volunteers were screened, of whom 210 were randomly assigned to receive either 35 or 305 bites of Ae. aegypti (n = 20 and n = 19, respectively), Ae. albopictus (n = 20, n = 21), An. dirus (n = 21, n = 21), An. maculatus (n = 23, n = 24), or An. minimus (n = 22, n = 19), comprising the intention-to-treat population. In participants exposed to 305 An. minimus bites, total anti-gSG6-P1 IgG levels increased 1.14-fold (95% confidence interval [CI] 1.03-1.26) and 1.18-fold (95% CI 1.05-1.33) during the exposure and post-exposure periods respectively (relative to baseline), with minimal or no boosting observed in other groups. The estimated half-life of anti-gSG6-P1 antibodies was 421 (95% CI 155-688) days. Seven participants were withdrawn due to an adverse event. CONCLUSIONS: Anti-gSG6-P1 antibodies were boosted in response to exposure to 305 bites of An. minimus, but the magnitude of boosting was small and antibodies decayed slowly. Future research is warranted to identify and validate serological markers of vector biting exposures. TRIAL REGISTRATION: The trial was registered in ClinicalTrials.gov: NCT04478370.