Role of Oxidative Stress-Dependent C/EBPβ Expression on CAF Transformation Inducing HCT116 Colorectal Cancer Cell Progression; Migration and Invasion
Issued Date
2023-11-01
Resource Type
eISSN
2476762X
Scopus ID
2-s2.0-85178498786
Pubmed ID
38019240
Journal Title
Asian Pacific journal of cancer prevention : APJCP
Volume
24
Issue
11
Start Page
3825
End Page
3835
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific journal of cancer prevention : APJCP Vol.24 No.11 (2023) , 3825-3835
Suggested Citation
Hassametto A., Tanomrat R., Muangthong T., Worawichawong S., Suwannalert P. Role of Oxidative Stress-Dependent C/EBPβ Expression on CAF Transformation Inducing HCT116 Colorectal Cancer Cell Progression; Migration and Invasion. Asian Pacific journal of cancer prevention : APJCP Vol.24 No.11 (2023) , 3825-3835. 3835. doi:10.31557/APJCP.2023.24.11.3825 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/91435
Title
Role of Oxidative Stress-Dependent C/EBPβ Expression on CAF Transformation Inducing HCT116 Colorectal Cancer Cell Progression; Migration and Invasion
Author's Affiliation
Other Contributor(s)
Abstract
OBJECTIVE: To investigate oxidative stress-related CAF transformation through C/EBPβ, which affects CRC progression and may have a potential implication for CRC treatment. METHODS: The conditioned media (CM) from HCT116, CRC cells, was used to activate CCD-18Co, colon fibroblasts, then the ability of activated FBs to induce HCT116 growth and progression was assessed using MTT assay, transwell migration, and matrix invasion assay. Alteration of the cytokine profile and oxidative stress of the activated FBs were studied with cytokine arrays and DCFH-DA assay, respectively. The protein expressions of the CAF markers (α-SMA and FAP) and C/EBPβ were investigated with immunofluorescence and western blotting. RESULT: It was found that CM from HCT116 cells induced oxidative stress, change of cytokine profile, CAF markers, and the C/EBPβ expression of activated FBs. Furthermore, when the oxidative stress of the activated FBs was suppressed, FAP and C/EBPβ expression were downregulated, correlating with the disabling of their capability to support the cancer progression. The C/EBPβ and prognosis for CRC patients were accessed using the GEPIA dataset, in which high C/EBPβ expression was associated with a poor prognosis. CONCLUSION: These findings suggest that C/EBPβ expression has a role in CAF transformation in an oxidative stress-related manner and might be used as a target to improve aggressive CRC treatment outcomes.