Development of a highly sensitive nucleic acid amplification-based detection for human leptospirosis infection
Issued Date
2022-06-01
Resource Type
ISSN
07366205
eISSN
19409818
Scopus ID
2-s2.0-85133928628
Pubmed ID
35698846
Journal Title
BioTechniques
Volume
73
Issue
1
Start Page
19
End Page
24
Rights Holder(s)
SCOPUS
Bibliographic Citation
BioTechniques Vol.73 No.1 (2022) , 19-24
Suggested Citation
Jiradechbadee T., Sawangjaroen N., Ranong G.N., Sila T., Buncherd H., Tun A.W., Thanapongpichat S. Development of a highly sensitive nucleic acid amplification-based detection for human leptospirosis infection. BioTechniques Vol.73 No.1 (2022) , 19-24. 24. doi:10.2144/btn-2021-0124 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87491
Title
Development of a highly sensitive nucleic acid amplification-based detection for human leptospirosis infection
Author's Affiliation
Other Contributor(s)
Abstract
Highly sensitive diagnostic tools are crucial for individual screening during an epidemic of leptospirosis. To aid in developing a diagnostic tool for the sensitive detection of pathogenic strains, a new approach targeting nucleic acid amplification that combines quantitative PCR (qPCR) and strand displacement isothermal amplification was evaluated. The effectiveness of the combined approach, a quantitative polymerase chain displacement reaction (qPCDR), was compared with a qPCR technique. The results showed that qPCDR presented higher sensitivity (at least tenfold) and shorter reaction time than the qPCR approach for pathogenic Leptospira spp. detection. Thus, the qPCDR-based technique developed in this study is a promising approach for pathogenic Leptospira spp. detection and the further development of a diagnostic kit.