Immunoproteomics to identify species-specific antigens in Neospora caninum recognised by infected bovine sera
Issued Date
2022-01-01
Resource Type
ISSN
1252607X
eISSN
17761042
Scopus ID
2-s2.0-85144578343
Pubmed ID
36562441
Journal Title
Parasite
Volume
29
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasite Vol.29 No.1 (2022)
Suggested Citation
Udonsom R., Reamtong O., Adisakwattana P., Popruk S., Jirapattharasate C., Nishikawa Y., Inpankaew T., Toompong J., Kotepui M., Mahittikorn A. Immunoproteomics to identify species-specific antigens in Neospora caninum recognised by infected bovine sera. Parasite Vol.29 No.1 (2022). doi:10.1051/parasite/2022059 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83354
Title
Immunoproteomics to identify species-specific antigens in Neospora caninum recognised by infected bovine sera
Other Contributor(s)
Abstract
Bovine neosporosis is a disease of concern due to its global distribution and significant economic impact through massive losses in the dairy and meat industries. To date, there is no effective chemotherapeutic drug or vaccine to prevent neosporosis. Control of this disease is therefore dependent on efficient detection tests that may affect treatment management strategies. This study was conducted to identify the specific immunoreactive proteins of Neospora caninum tachyzoites recognised by sera from cattle infected with N. caninum, Toxoplasma gondii, Cryptosporidium parvum, Babesia bovis and B. bigemina, and by sera from uninfected cattle using two-DE dimensional gel electrophoresis (2-DE) combined with immunoblot and mass spectrometry (LC-MS/MS). Among 70 protein spots that reacted with all infected sera, 20 specific antigenic spots corresponding to 14 different antigenic proteins were recognised by N. caninum-positive sera. Of these immunoreactive antigens, proteins involved in cell proliferation and invasion process were highly immunogenic, including HSP90-like protein, putative microneme 4 (MIC4), actin, elongation factor 1-alpha and armadillo/beta-catenin-like repeat-containing protein. Interestingly, we discovered an unnamed protein product, rhoptry protein (ROP1), possessing strong immunoreactivity against N. caninum but with no data on function available. Moreover, we identified cross-reactive antigens among these apicomplexan parasites, especially N. caninum, T. gondii and C. parvum. Neospora caninum-specific immunodominant proteins were identified for immunodiagnosis and vaccine development. The cross-reactive antigens could be evaluated as potential common vaccine candidates or drug targets to control the diseases caused by these apicomplexan protozoan parasites.