Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
Issued Date
2023-06-01
Resource Type
ISSN
09209069
eISSN
15730778
Scopus ID
2-s2.0-85150179509
Journal Title
Cytotechnology
Volume
75
Issue
3
Start Page
219
End Page
229
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cytotechnology Vol.75 No.3 (2023) , 219-229
Suggested Citation
Choa J.B.D., Sasaki T., Kajiura H., Ikuta K., Fujiyama K., Misaki R. Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells. Cytotechnology Vol.75 No.3 (2023) , 219-229. 229. doi:10.1007/s10616-023-00571-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81371
Title
Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of the medium is an effective approach to improving the production and quality of recombinant proteins. In this study, we adapted IgA1-producing CHO-K1 suspension cells to a high concentration (150 mM) of different disaccharides, namely sucrose, maltose, lactose, and trehalose, to improve the production and quality of recombinant IgA1. The disaccharide-adapted cell lines had slower cell growth rates, but their cell viability was extended compared to the nonadapted IgA1-producing cell line. Glucose consumption was exhausted in all cell lines except for the maltose-adapted one, which still contained glucose even after the 9th day of culturing. Lactate production was higher among the disaccharide-adapted cell lines. The specific productivity of the maltose-adapted IgA1-producing line was 4.5-fold that of the nonadapted line. In addition, this specific productivity was higher than in previous productions of recombinant IgA1 with a lambda chain. Lastly, secreted IgA1 aggregated in all cell lines, which may have been caused by self-aggregation. This aggregation was also found to begin inside the cells for maltose-adapted cell line. These results suggest that a high concentration of disaccharide-supplemented induced hyperosmolarity in the IgA1-producing CHO-K1 cell lines. In addition, the maltose-adapted CHO-K1 cell line benefited from having an additional source of carbohydrate.