Genome-wide gene expression profiles throughout human malaria parasite liver stage development in humanized mice
Issued Date
2025-01-01
Resource Type
eISSN
20585276
Scopus ID
2-s2.0-85217268281
Journal Title
Nature Microbiology
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nature Microbiology (2025)
Suggested Citation
Zanghí G., Patel H., Smith J.L., Camargo N., Bae Y., Hesping E., Boddey J.A., Venugopal K., Marti M., Flannery E.L., Chuenchob V., Fishbaugher M.E., Mikolajczak S.A., Roobsoong W., Sattabongkot J., Gupta P., Pazzagli L., Rezakhani N., Betz W., Hayes K., Goswami D., Vaughan A.M., Kappe S.H.I. Genome-wide gene expression profiles throughout human malaria parasite liver stage development in humanized mice. Nature Microbiology (2025). doi:10.1038/s41564-024-01905-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/105305
Title
Genome-wide gene expression profiles throughout human malaria parasite liver stage development in humanized mice
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
University of Washington School of Medicine
Walter and Eliza Hall Institute of Medical Research
University of Melbourne
University of Washington
Universität Zürich
Seattle Biomedical Research Institute
University of Glasgow
Novartis Biomedical Research
Seattle Children’s Research Institute
University of Washington School of Medicine
Walter and Eliza Hall Institute of Medical Research
University of Melbourne
University of Washington
Universität Zürich
Seattle Biomedical Research Institute
University of Glasgow
Novartis Biomedical Research
Seattle Children’s Research Institute
Corresponding Author(s)
Other Contributor(s)
Abstract
Gene expression of Plasmodium falciparum (Pf) liver-stage (LS) parasites has remained poorly characterized, although they are major vaccine and drug targets. Using a human liver-chimaeric mouse model and a fluorescent parasite line (PfNF54CSPGFP), we isolated PfLS and performed transcriptomics on key LS developmental phases. We linked clustered gene expression to ApiAP2, a major family of transcription factors that regulate the parasite life cycle. This provided insights into transcriptional regulation of LS infection and expression of essential LS metabolic and biosynthetic pathways. We observed expression of antigenically variant PfEMP1 proteins and the major Pf protein export machine PTEX and identified protein candidates that might be exported by LS parasites. Comparing Pf and P. vivax LS transcriptomes, we uncovered differences in their expression of sexual commitment factors. This data will aid LS research and vaccine and drug target identification for prevention of malaria infection.