Lactiplantibacillus plantarum dfa1 outperforms enterococcus faecium dfa1 on anti-obesity in high fat-induced obesity mice possibly through the differences in gut dysbiosis attenuation, despite the similar anti-inflammatory properties
Issued Date
2022-01-01
Resource Type
eISSN
20726643
Scopus ID
2-s2.0-85121651730
Pubmed ID
35010955
Journal Title
Nutrients
Volume
14
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nutrients Vol.14 No.1 (2022)
Suggested Citation
Ondee T., Pongpirul K., Janchot K., Kanacharoen S., Lertmongkolaksorn T., Wongsaroj L., Somboonna N., Ngamwongsatit N., Leelahavanichkul A. Lactiplantibacillus plantarum dfa1 outperforms enterococcus faecium dfa1 on anti-obesity in high fat-induced obesity mice possibly through the differences in gut dysbiosis attenuation, despite the similar anti-inflammatory properties. Nutrients Vol.14 No.1 (2022). doi:10.3390/nu14010080 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83397
Title
Lactiplantibacillus plantarum dfa1 outperforms enterococcus faecium dfa1 on anti-obesity in high fat-induced obesity mice possibly through the differences in gut dysbiosis attenuation, despite the similar anti-inflammatory properties
Other Contributor(s)
Abstract
Fat reduction and anti-inflammation are commonly claimed properties of probiotics. Lactiplan-tibacillus plantarum and Enterococcus faecium were tested in high fat-induced obesity mice and in vitro experiments. After 16 weeks of probiotics, L. plantarum dfa1 outperforms E. faecium dfa1 on the anti-obesity property as indicated by body weight, regional fat accumulation, serum cholesterol, inflammatory cytokines (in blood and colon tissue), and gut barrier defect (FITC-dextran assay). With fecal microbiome analysis, L. plantarum dfa1 but not E. faecium dfa1 reduced fecal abundance of pathogenic Proteobacteria without an alteration in total Gram-negative bacteria when compared with non-probiotics obese mice. With palmitic acid induction, the condition media from both probiotics similarly attenuated supernatant IL-8, improved enterocyte integrity and down-regulated cholesterol absorption-associated genes in Caco-2 cell (an enterocyte cell line) and reduced supernatant cytokines (TNF-α and IL-6) with normalization of cell energy status (extracellular flux analysis) in bone-marrow-derived macrophages. Due to the anti-inflammatory effect of the condition media of both probiotics on palmitic acid-activated enterocytes was neutralized by amylase, the active anti-inflammatory molecules might, partly, be exopolysaccharides. As L. plantarum dfa1 out-performed E. faecium dfa1 in anti-obesity property, possibly through the reduced fecal Proteobacteria, with a similar anti-inflammatory exopolysaccharide; L. plantarum is a potentially better option for anti-obesity than E. faecium.