Distribution of Epstein-Barr virus in the oral cavity of Thais with various oral mucosal conditions
Issued Date
2024-01-30
Resource Type
ISSN
24058440
Scopus ID
2-s2.0-85182368734
Journal Title
Heliyon
Volume
10
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.10 No.2 (2024)
Suggested Citation
Vorakulpipat P., Kitkumthorn N., Lapthanasupkul P., Rungraungrayabkul D., Klongnoi B., Khovidhunkit S.o.P. Distribution of Epstein-Barr virus in the oral cavity of Thais with various oral mucosal conditions. Heliyon Vol.10 No.2 (2024). doi:10.1016/j.heliyon.2024.e24222 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95817
Title
Distribution of Epstein-Barr virus in the oral cavity of Thais with various oral mucosal conditions
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Corresponding Author(s)
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Abstract
Objectives: We aimed to examine the presence of EBV, EBV strains, and variants among 3 oral conditions including normal oral mucosa (NOM), oral potentially malignant disorders/oral cancer (OPMDs/OC) and non-OPMDs/OC in a group of Thais. Material and methods: Oral exfoliated cells were obtained from 315 participants living in the northeastern and central regions of Thailand. The participants were divided into 3 groups encompassing the NOM, the OPMDs/OC and the non-OPMDs/OC groups. The presence of EBV was first determined by PCR using primers for LMP1 gene. Subsequently, EBV strains of EBNA3c and variants based on LMP1 sequences were determined by real-time PCR. Results: The prevalence of EBV in OPMDs/OC, non-OPMDs/OC and NOM were 72.0 %, 56.2 %, and 27.2 % respectively. EBV type A, B and AB were found in 52.1 %, 32.1 % and 15.8 % of all positive samples, respectively. The percentage of participants with EBV type A was more prominent in the NOM group (72.0 %) compared to the non-OPMDs/OC (54.8 %) and the OPMDs/OC group (41.8 %) whereas EBV type B was higher in the OPMDs/OC group (35.8 %) compared to the non-OPMDs/OC (31.5 %) and the NOM (24.0 %) groups. Regarding EBV variants, 30-bp deletion LMP1 variant (del-LMP1) which is more associated with malignant transformation was predominately found in the OPMDs/OC (32.8 %) and the non-OPMDs/OC (38.4 %) groups compared to the NOM group (20.0 %). Conclusions: High frequency of EBV was demonstrated in the OPMDs/OC group. EBV type A was more predominant in the NOM group whereas EBV type B was more prevalent in the OPMDs/OC group. The del-LMP1 variant was more common in the OPMDs/OC and the non-OPMDs/OC groups.