Assessment of therapeutic effect of CD20-targeted immunoliposome in primary central nervous system lymphoma
Issued Date
2022-06-01
Resource Type
ISSN
07533322
eISSN
19506007
Scopus ID
2-s2.0-85128691734
Pubmed ID
35461090
Journal Title
Biomedicine and Pharmacotherapy
Volume
150
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biomedicine and Pharmacotherapy Vol.150 (2022)
Suggested Citation
Thumrongsiri N., Dana P., Bawab R., Tanyapanyachon P., Treetidnipa C., Saengkrit N., Sathornsumetee S. Assessment of therapeutic effect of CD20-targeted immunoliposome in primary central nervous system lymphoma. Biomedicine and Pharmacotherapy Vol.150 (2022). doi:10.1016/j.biopha.2022.112979 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86861
Title
Assessment of therapeutic effect of CD20-targeted immunoliposome in primary central nervous system lymphoma
Author's Affiliation
Other Contributor(s)
Abstract
Primary central nervous system lymphoma (PCNSL) is a form of extranodal non-Hodgkin's B-cell lymphoma limited to the CNS. The treatment of PCNSL is ineffective partly due to the blood-brain barrier (BBB) restriction of delivery of many drugs including anti-CD20 (Rituximab; RTX) which is a standard treatment for systemic B-cell lymphomas. In this study, liposome with tween-80 surface modification was fabricated and conjugated with RTX for enhancing BBB penetration to target lymphoma cells in the CNS. Physicochemical characterizations of Lip/RTX were performed and spherical shape liposomes with narrow size distribution were demonstrated by TEM. An average diameter of Lip/RTX was 168.57 ± 1.57 nm with the percentage of RTX conjugation at 90.94. Cell internalization monitored by flow cytometry confirmed that conjugation of RTX promoted liposome entry into Raji cells expressing CD20. Antitumor activity of Lip/RTX was comparable to free RTX indicating that RTX moieties on liposome remained their therapeutic function. In addition, Lip/RTX inhibited tumor aggressiveness by limiting cell migration and invasion. Systemic administration of Lip/RTX significantly prolonged survival of mice harboring intracranial lymphoma xenografts. Taken together, Lip/RTX presents a new potential treatment for patients with PCNSL.