Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
Issued Date
2023-02-01
Resource Type
eISSN
24146366
Scopus ID
2-s2.0-85149201824
Journal Title
Tropical Medicine and Infectious Disease
Volume
8
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Medicine and Infectious Disease Vol.8 No.2 (2023)
Suggested Citation
Luvira V., Thawornkuno C., Lawpoolsri S., Thippornchai N., Duangdee C., Ngamprasertchai T., Leaungwutiwong P. Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique. Tropical Medicine and Infectious Disease Vol.8 No.2 (2023). doi:10.3390/tropicalmed8020117 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82402
Title
Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique
Author's Affiliation
Other Contributor(s)
Abstract
Dengue infection has been a public health problem worldwide, especially in tropical areas. A lack of sensitive diagnostic methods in the early phase of the illness is one of the challenging problems in clinical practices. We, herein, analyzed 86 sera of acute febrile patients, from both dengue and non-dengue febrile illness, to study the diagnostic performance of dengue diagnostics. When compared with detection by Polymerase Chain Reaction (PCR), dengue NS1 detection by enzyme-linked immunosorbent assay (ELISA) had the highest sensitivity of 82.4% (with 94.3% specificity), while NS1 by rapid diagnostic test (RDT) had 76.5% sensitivity. IgM detection by ELISA and RDT showed only 27.5% and 17.9% sensitivity, respectively. The combination of NS1 and IgM in RDT yielded a sensitivity of 78.4%, with 97.1% specificity. One of the essential steps in making a diagnosis from patient samples is the preparation process. At present, a variety of techniques have been used to increase the number of analytes in clinical samples. In this study, we focused on the sample concentration method. The sera were concentrated three times with the ultrafiltration method using a 10 kDa molecular weight cut-off membrane. The results showed an increase in the sensitivity of RDT-NS1 detection at 80.4%, with 100% specificity. When combining NS1 and IgM detection, the concentration method granted RDT an 82.4% sensitivity, with 100% specificity. In conclusion, serum concentration by the ultrafiltration method is a simple and applicable technique. It could increase the diagnostic performance of point-of-care dengue diagnostics.