Prevalence of asymptomatic genital herpes in near-term pregnant women and anti-HSV-2 IgM response following first episode and recurrent diseases
1
Issued Date
1988
Copyright Date
1988
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 93 leaves. : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Microbiology))--Mahidol University, 1988
Suggested Citation
Wanna Pumeechockchai Prevalence of asymptomatic genital herpes in near-term pregnant women and anti-HSV-2 IgM response following first episode and recurrent diseases. Thesis (M.Sc. (Microbiology))--Mahidol University, 1988. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103365
Title
Prevalence of asymptomatic genital herpes in near-term pregnant women and anti-HSV-2 IgM response following first episode and recurrent diseases
Alternative Title(s)
ความซุกของโรคติดเชื้อเริมที่อวัยวะสืบพันธุ์ชนิดไม่ปรากฎอาการในหญิงตั้งครรภ์ใกล้คลอด และภูมิคุ้มกันตอบสนองชนิด IgM ในผู้ที่ปรากฎอาการเป็นครั้งแรกและผู้ที่ติดเชื้อกลับซ้ำ
Author(s)
Advisor(s)
Abstract
Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), similar to other menbers of viruses in the family Herpetoviridae, can cause latent infection in their natural hosts. This infection can be reactivated and manifested with or without signs adn symptoms, referred to as symptomatic or asymptomatic infections, respectively. Genital herpes in pregnant women has been shown to affect fetuses and newborns and cause significant morbidity and mortality. Thus, cervido-vaginal swabs for detection of HSV shedding shold be done during the last month of gestation in women with a history of genital herpes. In the present study, the prevalence of asymptomatic genital herpes in near-term pregnant (at least 30 weeks of gestation) who attended the antenatal clinic at Ramathibodi Hospital was investigated by using in vitro cultivation procedure. Various other methods for detectionof HSV antigens were also compared to this standard cell culture assay. These includes biotin - streptavidin enzyme-linked immunosorbent assay (B-SA ELISA), immunoperoxidase staining of tissue smears and the staphylococcal coagglutination test. An attempt to detect anti-HSV-2 IgM was also carried out in cord blood samples obtained from the infants born to some of these mothers. Anit-HSV-2 Igm response was also determined in adults with genital herpetic lesions. The results indicatied that the prevalence of asymptomatic genital herpes infection in these pregnant women was very low, i.e, 1 of 808 (0.12%) was positive by viral isolation and B-SA ELISA while samples obtained from 2 pregnant women with herpetic lesions were positive by both tests. The protocol of B-SA ELISA used in the present study can detect both HSV-1 and HSV-2 but not varicella-zoster and cytomegalo-virus. The value of immunoperoxidase staining of tissue smears cannot be evaluated since specimens from pregnant women who excreted the virus are unavailable. Staphylococcal co-agglutination test could not detect HSV antigens in all 3 HSV-isolation and B-SA ELISA positive samples. When the sensitivity of this asssy technique for detectionof HSV was determined, it was found to be about 800-fold less sensitive than the B-SA ELISA. It could detect only 6 of 125 (4.8%) whereas 87 of 125 (69.6%) and 84 of 125 (67.2%) was positive by HSV isolation and by B-SA ELISA, respectively. Thus, B-SA ELISA seems to be the most sensitive immunological assay for HSV detection. The assay can be more rapid if either a suitable amonut of PEG is included in the detecting antibodies and streptavidin peroxidase of high concentrations of both reagents were used. For detection of anti-HSV-2 IgM, the diluent should contain high salt concentration (i.e., 0.5 m NaCI) to reduce non-specific binding to the solid phase. The results indicated that anti-HSV-2 LgM was not detectable in all cord sera including from all infants born to 3 monther who shed HSV during gestation. However, this assay protocol can detect anti-HSV-2 IgM in ll of 122 (9.0%) and 23 of 141 (16.3%) sera from the patients with lst episode (geometric mean titer of 1:5,558) and recurrent genital herpes (geometric mean titer of 1:680), respectively. It can be then concluded that anti-HSV-2 IgM should not be used to differentiate between 1st episode and recurrent genital herpes infection in adults, although it can be used to notify HSV infection in newborn infants. In addition, infants may acquire infection intrapartum or postnatally, thus serum samples for anti-HSV IgM detection should be obtained during 1-month period after birth.
Description
Microbiology (Mahidol University 1988)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Microbiology
Degree Grantor(s)
Mahidol University