Cadmium-resistant Streptomyces stimulates phytoextraction potential of Crotalaria juncea L. in cadmium-polluted soil

Abstract

This work evaluated the competence of two strains of cadmium (Cd)-resistant Streptomyces, namely Streptomyces rapamycinicus K5PN1, an indole-3-acetic acid (IAA) producer, and Streptomyces cyaneus 11-10SHTh, a siderophore producer on promoting Cd phytoextraction by sunn hemp. The results showed that S. rapamycinicus improved root elongation of sunn hemp seedlings under Cd stress conditions. S. rapamycinicus and S. cyaneus were colonized on the root surface of sunn hemp at concentrations of 2.3 × 104 and 6.4 × 103 CFU g−1 root fresh weight, respectively. The results of pot-culture experiments showed that S. rapamycinicus increased the root and shoot lengths, and dry biomass of sunn hemp planted in high Cd-contaminated soil. The Cd concentration in the leaves of sunn hemp inoculated with S. cyaneus (73.82 ± 2.20 mg kg−1 plant dry wt) was higher than that of plants with S. rapamycinicus inoculation and the uninoculated control. The phytoextraction of Cd by sunn hemp was significantly increased with Cd-resistant Streptomyces inoculation. In conclusion, both strains of Cd-resistant Streptomyces had potential on enhancing Cd phytoextraction efficiency of sunn hemp. Our study suggests the application of Cd-resistant Streptomyces can improve Cd phytoextraction by sunn hemp for restoration of Cd-polluted sites.