Overcoming addition of phosphoethanolamine to lipid A mediated colistin resistance in Acinetobacter baumannii clinical isolates with colistin–sulbactam combination therapy
Issued Date
2022-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85133508320
Pubmed ID
35794134
Journal Title
Scientific Reports
Volume
12
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.12 No.1 (2022)
Suggested Citation
Srisakul S., Wannigama D.L., Higgins P.G., Hurst C., Abe S., Hongsing P., Saethang T., Luk-in S., Liao T., Kueakulpattana N., Shein A.M.S., Gan L., Kupwiwat R., Tanasatitchai C., Wapeesittipan P., Phattharapornjaroen P., Badavath V.N., Leelahavanichkul A., Chatsuwan T. Overcoming addition of phosphoethanolamine to lipid A mediated colistin resistance in Acinetobacter baumannii clinical isolates with colistin–sulbactam combination therapy. Scientific Reports Vol.12 No.1 (2022). doi:10.1038/s41598-022-15386-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86409
Title
Overcoming addition of phosphoethanolamine to lipid A mediated colistin resistance in Acinetobacter baumannii clinical isolates with colistin–sulbactam combination therapy
Author's Affiliation
Yamagata Prefectural Central Hospital
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Narsee Monjee Institute of Management Studies, Mumbai
Universitetet i Bergen
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Kasetsart University
Mae Fah Luang University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Thammasat University
Faculty of Medicine, Chulalongkorn University
Partner Site Bonn-Cologne
Fuling Center Hospital of Chongqing City
Medizinische Fakultät
Mae Fah Luang University Hospital
UWA Medical School
Narsee Monjee Institute of Management Studies, Mumbai
Universitetet i Bergen
Chulalongkorn University
Sahlgrenska Akademin
QIMR Berghofer Medical Research Institute
Kasetsart University
Mae Fah Luang University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Mahidol University
Thammasat University
Faculty of Medicine, Chulalongkorn University
Partner Site Bonn-Cologne
Fuling Center Hospital of Chongqing City
Other Contributor(s)
Abstract
Overcoming colistin-resistant Acinetobacter baumannii (CoR-AB) has become a major concern due to the lack of effective antibiotics. This study aimed to explore the prevalence of CoR-AB clinical isolates in Thailand, their mechanisms of resistance, and test the efficacy of colistin plus sulbactam against CoR-AB isolates. The colistin resistance rate among carbapenem-resistant A. baumannii was 15.14%. The mcr gene or its variants were not detected in CoR-AB isolates by PCR screening. The lipid A mass spectra of CoR-AB isolates showed the additional [M–H]− ion peak at m/z = 2034 that correlated to the phosphoethanolamine (pEtN) addition to lipid A (N = 27/30). The important amino acid substitutions were found at position S14P, A138T, A227V in PmrB that are associated with overexpression of the pEtN transferase (PmrC) and contributed the pEtN addition. The lipopolysacccharide production genes (lpxACD) were not related to lipid A mass spectra. A colistin plus sulbactam combination exhibited the synergy rate at 86.7% against CoR-AB isolates compare to sulbactam (85.89% resistance) or colistin (15.14% resistance) alone. The excellent synergistic activity of colistin plus sulbactam combination has the potential for the treatment of CoR-AB infections.