Occlusive and Proliferative Properties of Different Collagen Membranes—An In Vitro Study
2
Issued Date
2023-02-01
Resource Type
eISSN
19961944
Scopus ID
2-s2.0-85149202808
Journal Title
Materials
Volume
16
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Materials Vol.16 No.4 (2023)
Suggested Citation
Sehgal V., Ruangsawasdi N., Kiattavorncharoen S., Bencharit S., Thanasrisuebwong P. Occlusive and Proliferative Properties of Different Collagen Membranes—An In Vitro Study. Materials Vol.16 No.4 (2023). doi:10.3390/ma16041657 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81990
Title
Occlusive and Proliferative Properties of Different Collagen Membranes—An In Vitro Study
Author's Affiliation
Other Contributor(s)
Abstract
Different collagen barrier membranes come in various sources and crosslinking that may affect barrier function and tissue integration. This study investigated barrier function and tissue integration of the three different collagen membranes (Jason®: porcine pericardium, GENOSS: bovine tendon, and BioMend® Extend: cross-linked bovine tendon) with human gingival fibroblasts. The barrier function and tissue integration properties were determined under confocal microscopy. Morphological characteristics were observed using scanning electron microscopy. Our results showed that all collagen membranes allowed a small number of cells to migrate, and the difference in barrier function ability was not significant. The cross-linked characteristics did not improve barrier ability. The native collagen membrane surfaces allowed evenly scattered proliferation of HGF, while the cross-linked collagen membrane induced patchy proliferation. Statistically significant differences in cell proliferation were found between Jason and BioMend Extend membranes (p = 0.04). Scanning electron microscope showed a compact membrane surface at the top, while the bottom surfaces displayed interwoven collagen fibers, which were denser in the crosslinked collagen membranes. Within the limitations of this study, collagen membranes of different origins and physical properties can adequately prevent the invasion of unwanted cells. Native collagen membranes may provide a better surface for gingival cell attachment and proliferation.